LOCUS pBAC-GMR 11231 bp DNA circular 28-OCT-2005 SOURCE ORGANISM COMMENT This file is created by Vector NTI http://www.informaxinc.com/ COMMENT VNTDATE|388084108| COMMENT VNTDBDATE|389526878| COMMENT VNTNAME|pBAC-GMR| COMMENT VNTAUTHORNAME|Maxim Koriabine| COMMENT VNTAUTHOREML|mkoriabine@chori.org| COMMENT VNTAUTHORWWW|http://bacpac.chori.org| COMMENT VNTOAUTHORNAME|UNKNOWN| COMMENT VNTREPLTYPE|Plasmid COMMENT VNTEXTCHREPL|Bacteria FEATURES Location/Qualifiers CDS 2865..4283 /vntifkey="4" /label=SacB misc_feature 1..2741 /vntifkey="21" /label=pUC-Link CDS complement(6279..7454) /vntifkey="4" /label=parA CDS complement(5308..6279) /vntifkey="4" /label=parB CDS complement(8033..8788) /vntifkey="4" /label=repE misc_feature 10999..11035 /vntifkey="21" /label=PI-SceI misc_feature 2748..2765 /vntifkey="21" /label=SP6 misc_feature 11208..11230 /vntifkey="21" /label=T7 promoter 11159..11199 /vntifkey="30" /label=SacB\gene\promoter CDS 10128..10787 /vntifkey="4" /label=CM(R) misc_feature 4718..5235 /vntifkey="21" /label=parC rep_origin 9127..9182 /vntifkey="33" /label=oriS misc_feature complement(9562..9909) /vntifkey="21" /label=redF misc_feature 11036..11060 /vntifkey="21" /label=attB1 misc_feature complement(2786..2810) /vntifkey="21" /label=attB2 BASE COUNT 3179 a 2707 c 2617 g 2728 t ORIGIN 1 gatctccgac gaattcgagc tcacgcgtac tgatgcatga tccgggttta aacccagtac 61 tctagatcct ctagagtcga cctgcaggca tgcaagcttg gcgtaatcat ggtcatagct 121 gtttcctgtg tgaaattgtt atccgctcac aattccacac aacatacgag ccggaagcat 181 aaagtgtaaa gcctggggtg cctaatgagt gagctaactc acattaattg cgttgcgctc 241 actgcccgct ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa tcggccaacg 301 cgcggggaga ggcggtttgc gtattgggcg ctcttccgct tcctcgctca ctgactcgct 361 gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt 421 atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc 481 caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga 541 gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata 601 ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac 661 cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg 721 taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc 781 cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag 841 acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt 901 aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta gaaggacagt 961 atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg 1021 atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac 1081 gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca 1141 gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac 1201 ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac 1261 ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt 1321 tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt 1381 accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt 1441 atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc 1501 cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa 1561 tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg 1621 tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt 1681 gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc 1741 agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt 1801 aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg 1861 gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac 1921 tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc 1981 gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt 2041 tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg 2101 aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat attattgaag 2161 catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa 2221 acaaataggg gttccgcgca catttccccg aaaagtgcca cctgacgtct aagaaaccat 2281 tattatcatg acattaacct ataaaaatag gcgtatcacg aggccctttc gtctcgcgcg 2341 tttcggtgat gacggtgaaa acctctgaca catgcagctc ccggagacgg tcacagcttg 2401 tctgtaagcg gatgccggga gcagacaagc ccgtcagggc gcgtcagcgg gtgttggcgg 2461 gtgtcggggc tggcttaact atgcggcatc agagcagatt gtactgagag tgcaccatat 2521 gcggtgtgaa ataccgcaca gatgcgtaag gagaaaatac cgcatcaggc gccattcgcc 2581 attcaggctg cgcaactgtt gggaagggcg atcggtgcgg gcctcttcgc tattacgcca 2641 gctggcgaaa gggggatgtg ctgcaaggcg attaagttgg gtaacgccag ggttttccca 2701 gtcacgacgt tgtaaaacga cggccagtga attcgttgga gatccttcta tagtgtcacc 2761 taaatgtcga cggccaggcg gccgcaccca gctttcttgt acaaagtggt gaggatcgaa 2821 acggcagatc gcaaaaaaca gtacatacag aaggagacat gaacatgaac atcaaaaaaa 2881 ttgtaaaaca agccacagtt ctgactttta cgactgcact tctggcagga ggagcgactc 2941 aagccttcgc gaaagaaaat aaccaaaaag catacaaaga aacgtacggc gtctctcata 3001 ttacacgcca tgatatgctg cagatcccta aacagcagca aaacgaaaaa taccaagtgc 3061 ctcaattcga tcaatcaacg attaaaaata ttgagtctgc aaaaggactt gatgtgtggg 3121 acagctggcc gctgcaaaac gctgacggaa cagtagctga atacaacggc tatcacgttg 3181 tgtttgctct tgcgggaagc ccgaaagacg ctgatgacac atcaatctac atgttttatc 3241 aaaaggtcgg cgacaactca atcgacagct ggaaaaacgc gggccgtgtc tttaaagaca 3301 gcgataagtt cgacgccaac gatccgatcc tgaaagatca gacgcaagaa tggtccggtt 3361 ctgcaacctt tacatctgac ggaaaaatcc gtttattcta cactgactat tccggtaaac 3421 attacggcaa acaaagcctg acaacagcgc aggtaaatgt gtcaaaatct gatgacacac 3481 tcaaaatcaa cggagtggaa gatcacaaaa cgatttttga cggagacgga aaaacatatc 3541 agaacgttca gcagtttatc gatgaaggca attatacatc cggcgacaac catacgctga 3601 gagaccctca ctacgttgaa gacaaaggcc ataaatacct tgtattcgaa gccaacacgg 3661 gaacagaaaa cggataccaa ggcgaagaat ctttatttaa caaagcgtac tacggcggcg 3721 gcacgaactt cttccgtaaa gaaagccaga agcttcagca gagcgctaaa aaacgcgatg 3781 ctgagttagc gaacggcgcc ctcggtatca tagagttaaa taatgattac acattgaaaa 3841 aagtaatgaa gccgctgatc acttcaaaca cggtaactga tgaaatcgag cgcgcgaatg 3901 ttttcaaaat gaacggcaaa tggtacttgt tcactgattc acgcggttca aaaatgacga 3961 tcgatggtat taactcaaac gatatttaca tgcttggtta tgtatcaaac tctttaaccg 4021 gcccttacaa gccgctgaac aaaacggggc ttgtgctgca aatgggtctt gatccaaacg 4081 atgtgacatt cacttactct cacttcgcag tgccgcaagc caaaggcaac aatgtggtta 4141 tcacaagcta catgacaaac agaggcttct tcgaggataa aaaggcaaca tttgcgccaa 4201 gcttcttaat gaacatcaaa ggcaataaaa catccgttgt caaaaacagc atcctggagc 4261 aaggacagct gacagtcaac taataacagc aaaaagaaaa tgccgatact tcattggcat 4321 tttcttttat ttctcaacaa gatggtgaat tgactagtgg gtagatccac aggacgggtg 4381 tggtcgccat gatcgcgtag tcgatagtgg ctccaagtag cgaagcgagc aggactgggc 4441 ggcggccaaa gcggtcagac agtgctccga gaacgggtgc gcatagaaat tgcatcaacg 4501 catatagcgc tagctcggaa tggacgatat cccgcgacag cgacacactt gcgttaacgt 4561 gccggcacgg cctgggtaac caggtatttt gtccacataa ccgtgcgcaa aatgctgtgg 4621 ataagcagga cacagcagca atccacagca ggcatacaac cgcacaccga ggttactccg 4681 ttctacaggt tacgacgaca tgtcaatact tgcccttgac aggcattgat ggaatcgtag 4741 tctcacgctg atagtctgat cgacaataca agtgggaccg tggtcccaga ccgataatca 4801 gaccgacaac acgagtggga tcgtggtccc agactaataa tcagaccgac gatacgagtg 4861 ggaccgtggt cccagactaa taatcagacc gacgatacga gtgggaccgt ggttccagac 4921 taataatcag accgacgata cgagtgggac cgtggtccca gactaataat cagaccgacg 4981 atacgagtgg gaccatggtc ccagactaat aatcagaccg acgatacgag tgggaccgtg 5041 gtcccagtct gattatcaga ccgacgatac gagtgggacc gtggtcccag actaataatc 5101 agaccgacga tacgagtggg accgtggtcc cagactaata atcagaccga cgatacgagt 5161 gggaccgtgg tcccagtctg attatcagac cgacgataca agtggaacag tgggcccaga 5221 gagaatattc aggccagtta tgctttctgg cctgtaacaa aggacattaa gtaaagacag 5281 ataaacgtag actaaaacgt ggtcgcatca gggtgctggc ttttcaagtt ccttaagaat 5341 ggcctcaatt ttctctatac actcagtagg aacacgagac ctgtccaggt taagcaccat 5401 tttatcgccc ttatacaata ctgtcgctcc aggagcaaac tgatgtcgtg agcttaaact 5461 agttcttgat gcagatgacg ttttaagcac agaagttaaa agagtgataa cttcttcagc 5521 ttcaaatatc accccagctt ttttctgctc atgaaggtta gatgcctgct gcttaagtaa 5581 ttcctcttta tctgtaaagg ctttttgaag tgcatcacct gaccgggcag atagttcacc 5641 ggggtgagaa aaaagagcaa caactgattt aggcaatttg gcggtgttga tacagcgggt 5701 aataatctta cgtgaaatat tttccgcatc agccagcgca gaaatatttc cagcaaattc 5761 attctgcaat cggcttgcat aacgctgacc acgttcataa gcacttgttg ggcgataatc 5821 gttacccaat ctggataatg cagccatctg ctcatcatcc agctcgccaa ccagaacacg 5881 ataatcactt tcggtaagtg cagcagcttt acgacggcga ctcccatcgg caatttctat 5941 gacaccagat actcttcgac cgaacgccgg tgtctgttga ccagtcagta gaaaagaagg 6001 gatgagatca tccagtgcgt cctcagtaag cagctcctgg tcacgttcat tacctgacca 6061 tacccgagag gtcttctcaa cactatcacc ccggagcact tcaagagtaa acttcacatc 6121 ccgaccacat acaggcgaag taatggcatt accgcgagcc attactccta cgcgcgcaat 6181 taacgaatcc accatcgggg cagctggtgt cgataacgaa gtatcttcaa ccggttgagt 6241 attgagcgta tgttttggaa taacaggcgc acgcttcatt atctaatctc ccagcgtggt 6301 ttaatcagac gatcgaaaat ttcattgcag acaggttccc aaatagaaag agcatttctc 6361 caggcaccag ttgaagagcg ttgatcaatg gcctgttcaa aaacagttct catccggatc 6421 tgacctttac caacttcatc cgtttcacgt acaacatttt ttagaaccat gcttccccag 6481 gcatcccgaa tttgctcctc catccacggg gactgagagc cattactatt gctgtatttg 6541 gtaagcagaa tacgtacatc aggctcgaac cctttaagat caacgttctt gagcagatca 6601 cgaagcatat cgaaaaactg cagtgcggag gtgtagtcaa acaactcagc aggcgtggga 6661 acgatcagca catcagcagc acatacgaca ttaatcgtgc cgatacccag gttaggcgcg 6721 ctgtcaataa ctatgacatc atagtcatga gcaacagttt caatggccag acggagcatc 6781 aggtgtggat cggtgggcag tttaccttca tcaaatttgc ccattaactc agtttcaata 6841 cggtgcagag ccagacagga aggaataatg tcaagccccg gccagcaagt gggctttatt 6901 gcataagtga catcgtcctt ttccccaaga tagaaaggca ggagagtgtc ttctgcatga 6961 atatgaagat ctggtaccca tccgtgatac attgaggctg ttccctgggg gtcgttacct 7021 tccacgagca aaacacgtag ccccttcaga gccagatcct gagcaagatg aacagaaact 7081 gaggttttgt aaacgccacc tttatgggca gcaaccccga tcaccggtgg aaatacgtct 7141 tcagcacgtc gcaatcgcgt accaaacaca tcacgcatat gattaatttg ttcaattgta 7201 taaccaacac gttgctcaac ccgtcctcga atttccatat ccgggtgcgg tagtcgccct 7261 gctttctcgg catctctgat agcctgagaa gaaaccccaa ctaaatccgc tgcttcacct 7321 attctccagc gccgggttat tttcctcgct tccgggctgt catcattaaa ctgtgcaatg 7381 gcgatagcct tcgtcatttc atgaccagcg tttatgcact ggttaagtgt ttccatgagt 7441 ttcattctga acatccttta atcattgctt tgcgtttttt tattaaatct tgcaatttac 7501 tgcaaagcaa caacaaaatc gcaaagtcat caaaaaaccg caaagttgtt taaaataaga 7561 gcaacactac aaaaggagat aagaagagca catacctcag tcacttatta tcactagcgc 7621 tcgccgcagc cgtgtaaccg agcatagcga gcgaactggc gaggaagcaa agaagaactg 7681 ttctgtcaga tagctcttac gctcagcgca agaagaaata tccaccgtgg gaaaaactcc 7741 aggtagaggt acacacgcgg atagccaatt cagagtaata aactgtgata atcaaccctc 7801 atcaatgatg acgaactaac ccccgatatc aggtcacatg acgaagggaa agagaaggaa 7861 atcaactgtg acaaactgcc ctcaaatttg gcttccttaa aaattacagt tcaaaaagta 7921 tgagaaaatc catgcaggct gaaggaaaca gcaaaactgt gacaaattac cctcagtagg 7981 tcagaacaaa tgtgacgaac caccctcaaa tctgtgacag ataaccctca gactatcctg 8041 tcgtcatgga agtgatatcg cggaaggaaa atacgatatg agtcgtctgg cggcctttct 8101 ttttctcaat gtatgagagg cgcattggag ttctgctgtt gatctcatta acacagacct 8161 gcaggaagcg gcggcggaag tcaggcatac gctggtaact ttgaggcagc tggtaacgct 8221 ctatgatcca gtcgattttc agagagacga tgcctgagcc atccggctta cgatactgac 8281 acagggattc gtataaacgc atggcatacg gattggtgat ttcttttgtt tcactaagcc 8341 gaaactgcgt aaaccggttc tgtaacccga taaagaaggg aatgagatat gggttgatat 8401 gtacactgta aagccctctg gatggactgt gcgcacgttt gataaaccaa ggaaaagatt 8461 catagccttt ttcatcgccg gcatcctctt cagggcgata aaaaaccact tccttccccg 8521 cgaaactctt caatgcctgc cgtatatcct tactggcttc cgcagaggtc aatccgaata 8581 tttcagcata tttagcaaca tggatctcgc agataccgtc atgttcctgt agggtgccat 8641 cagattttct gatctggtca acgaacagat acagcatacg tttttgatcc cgggagagac 8701 tatatgccgc ctcagtgagg tcgtttgact ggacgattcg cgggctattt ttacgtttct 8761 tgtgattgat aaccgctgtt tccgccatga cagatccatg tgaagtgtga caagttttta 8821 gattgtcaca ctaaataaaa aagagtcaat aagcagggat aactttgtga aaaaacagct 8881 tcttctgagg gcaatttgtc acagggttaa gggcaatttg tcacagacag gactgtcatt 8941 tgagggtgat ttgtcacact gaaagggcaa tttgtcacaa caccttctct agaaccagca 9001 tggataaagg cctacaaggc gctctaaaaa agaagatcta aaaactataa aaaaaataat 9061 tataaaaata tccccgtgga taagtggata accccaaggg aagttttttc aggcatcgtg 9121 tgtaagcaga atatataagt gctgttccct ggtgcttcct cgctcactcg agggcttcgc 9181 cctgtcgctc aactgcggcg agcactactg gctgtaaaag gacagaccac atcatggttc 9241 tgtgttcatt aggttgttct gtccattgct gacataatcc gctccacttc aacgtaacac 9301 cgcacgaaga tttctattgt tcctgaaggc atattcaaat cgttttcgtt accgcttgca 9361 ggcatcatga cagaacacta cttcctataa acgctacaca ggctcctgag attaataatg 9421 cggatctcta cgataatggg agattttccc gactgtttcg ttcgcttctc agtggataac 9481 agccagcttc tctgtttaac agacaaaaac agcatatcca ctcagttcca catttccata 9541 taaaggccaa ggcatttatt ctcaggataa ttgtttcagc atcgcaaccg catcagactc 9601 cggcatcgca aactgcaccc ggtgccgggc agccacatcc agcgcaaaaa ccttcgtgta 9661 gacttccgtt gaactgatgg acttatgtcc catcaggctt tgcagaactt tcagcggtat 9721 accggcatac agcatgtgca tcgcatagga atggcggaac gtatgtggtg tgaccggaac 9781 agagaacgtc acaccgtcag cagcagcggc ggcaaccgcc tccccaatcc aggtcctgac 9841 cgttctgtcc gtcacttccc agatccgcgc tttctctgtc cttcctgtgc gacggttacg 9901 ccgctccatg agcttatcgc gaataaatac ctgtgacgga agatcacttc gcagaataaa 9961 taaatcctgg tgtccctgtt gataccggga agccctgggc caacttttgg cgaaaatgag 10021 acgttgatcg gcacgtaaga ggttcctact ttcaccataa tgaaataaga tcactaccgg 10081 gcgtattttt tgagttatcg agattttcag gagctaagga agctaaaatg gagaaaaaaa 10141 tcactggata taccaccgtt gatatatccc aatggcatcg taaagaacat tttgaggcat 10201 ttcagtcagt tgctcaatgt acctataacc agaccgttca gctggatatt acggcctttt 10261 taaagaccgt aaagaaaaat aagcacaagt tttatccggc ctttattcac attcttgccc 10321 gcctgatgaa tgctcatccg gagttccgta tggcaatgaa agacggtgag ctggtgatat 10381 gggatagtgt tcacccttgt tacaccgttt tccatgagca aactgaaacg ttttcatcgc 10441 tctggagtga ataccacgac gatttccggc agtttctaca catatattcg caagatgtgg 10501 cgtgttacgg tgaaaacctg gcctatttcc ctaaagggtt tattgagaat atgtttttcg 10561 tctcagccaa tccctgggtg agtttcacca gttttgattt aaacgtggcc aatatggaca 10621 acttcttcgc ccccgttttc accatgggca aatattatac gcaaggcgac aaggtgctga 10681 tgccgctggc gattcaggtt catcatgccg tttgtgatgg cttccatgtc ggcagaatgc 10741 ttaatgaatt acaacagtac tgcgatgagt ggcagggcgg ggcgtaattt ttttaaggca 10801 gttattggtg cccttaaacg cctggttgct acgcctgaat aagtgataat aagcggatga 10861 atggcagaaa ttcgatgata agctgtcaaa catgagaatt ggtcgacggc gcgccaaagc 10921 ttgcatgcct gcagccgcgt aacctggcaa aatcggttac ggttgagtaa taaatggatg 10981 ccctgcgtaa gcggggcaca tttcattacc tctttctccg cacccgacat agataacaag 11041 tttgtacaaa aaagcaggct ctagtagact taattaagga tcgatccggc gcgccaatag 11101 tcatgccccg cgcccaccgg aaggagctga ctgggttgaa ggctctcaag ggcatcggtc 11161 gagcttgaca ttgtaggact atattgctct aataaatttg cggccgctaa tacgactcac 11221 tatagggaga g //