LOCUS pTARBAC4\( 13466 bp DNA circular 6-MAR-2001 DEFINITION pTARBAC4 is derived from pBACe3.6 by insertion of CEN6-His3 sequence at #9413bp, the XhoI site is gone. KEYWORDS PIETER DE JONG. SOURCE ORGANISM COMMENT This file is created by Vector NTI http://www.informaxinc.com/ COMMENT VNTDBDATE|224522179| COMMENT VNTNAME|pTARBAC4 (updated)| COMMENT VNTREPLTYPE|Plasmid COMMENT VNTEXTCHREPL|Bacteria COMMENT VNTEXTCHREPL|Yeast COMMENT Vector_NTI_Display_Data_(Do_Not_Edit!) 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0.075 1) COMMENT #274=(CLabel COMMENT (CWidget 0 (0 0) 1 2 0 0 #134# -703 100) COMMENT (LOGPEN 0 0 0) 1 COMMENT (LOGFONT 32 12 0 0 400 0 0 0 0 7 48 2 18 COMMENT "Times New Roman") 0.8 0.7 0 COMMENT "HindIII (13139)" "@N (@S)" 65535 -7.89909 COMMENT 10.4 0 -10 3.24786 0.564103 #273#))))) COMMENT #275=(CGroupWidget (CWidget 14 (16 0) 1 2 0 0 Nil 0 100) COMMENT (CObjectList)) COMMENT #276=(CGroupWidget (CWidget 12 (0 0) 1 2 0 0 Nil 0 100) COMMENT (CObjectList))))) COMMENT (CSeqView 10 5 (CObjectList) (CObList) 1 (CObList)) (CObList) 0 COMMENT (CStringList) 0 0 (CObList))) FEATURES Location/Qualifiers rep_origin complement(9249..9328) /vntifkey="33" /label=ORI /note="The minimum sequence required for replication (see Acc. # J01724)" misc_feature complement(2818..2835) /vntifkey="21" /label=SP6 misc_feature 13438..13460 /vntifkey="21" /label=T7 /note="T7 primer" CDS 2935..4353 /vntifkey="4" /label=SACBII /note="E.coli SacB gene" misc_feature 4839..4862 /vntifkey="21" /label=LOXP /note="LoxP (This is not the original LoxP site)" misc_feature complement(13257..13290) /vntifkey="21" /label=LOXP511 misc_feature complement(13220..13256) /vntifkey="21" /label=PI_SCEI misc_feature complement(13155..13219) /vntifkey="21" /label=TN7ATT misc_feature 4946..13131 /vntifkey="21" /label=PBAC108L /note="Fragment from mel Simons pBAC108L" misc_feature 37..2775 /vntifkey="21" /label=PUC-LINK misc_feature 9542..9882 /vntifkey="21" /label=Yeast\CEN6 CDS 9883..11372 /vntifkey="4" /label=Yeast\His3\cassette CDS 12141..13096 /vntifkey="4" /label=CM(R) BASE COUNT 3801 a 3175 c 3128 g 3362 t ORIGIN 1 gatccgcgga attcgagctc acgcgtactg atgcatgatc cgggtttaaa cccagtactc 61 tagatcctct agagtcgacc tgcaggcatg caagcttggc gtaatcatgg tcatagctgt 121 ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa catacgagcc ggaagcataa 181 agtgtaaagc ctggggtgcc taatgagtga gctaactcac attaattgcg ttgcgctcac 241 tgcccgcttt ccagtcggga aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg 301 cggggagagg cggtttgcgt attgggcgct cttccgcttc ctcgctcact gactcgctgc 361 gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat 421 ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca 481 ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc 541 atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc 601 aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg 661 gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcaatgc tcacgctgta 721 ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg 781 ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac 841 acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag 901 gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga aggacagtat 961 ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat 1021 ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc 1081 gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt 1141 ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct 1201 agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt 1261 ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc 1321 gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac 1381 catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat 1441 cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg 1501 cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata 1561 gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta 1621 tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt 1681 gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag 1741 tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa 1801 gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc 1861 gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt 1921 taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc 1981 tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta 2041 ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa 2101 taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca 2161 tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac 2221 aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgacgtctaa gaaaccatta 2281 ttatcatgac attaacctat aaaaataggc gtatcacgag gccctttcgt ctcgcgcgtt 2341 tcggtgatga cggtgaaaac ctctgacaca tgcagctccc ggagacggtc acagcttgtc 2401 tgtaagcgga tgccgggagc agacaagccc gtcagggcgc gtcagcgggt gttggcgggt 2461 gtcggggctg gcttaactat gcggcatcag agcagattgt actgagagtg caccatatgc 2521 ggtgtgaaat accgcacaga tgcgtaagga gaaaataccg catcaggcgc cattcgccat 2581 tcaggctgcg caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc 2641 tggcgaaagg gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt 2701 cacgacgttg taaaacgacg gccagtgaat tcgagctcgg tacccgggga tctagagtac 2761 tgggtttaaa cccggatcat gcatcagtac gcgtgagctc gaattccgcg gatccttcta 2821 tagtgtcacc taaatgtcga cggccaggcg gccgccaggc ctacccacta gtcaattcgg 2881 gaggatcgaa acggcagatc gcaaaaaaca gtacatacag aaggagacat gaacatgaac 2941 atcaaaaaaa ttgtaaaaca agccacagtt ctgactttta cgactgcact tctggcagga 3001 ggagcgactc aagccttcgc gaaagaaaat aaccaaaaag catacaaaga aacgtacggc 3061 gtctctcata ttacacgcca tgatatgctg cagatcccta aacagcagca aaacgaaaaa 3121 taccaagtgc ctcaattcga tcaatcaacg attaaaaata ttgagtctgc aaaaggactt 3181 gatgtgtggg acagctggcc gctgcaaaac gctgacggaa cagtagctga atacaacggc 3241 tatcacgttg tgtttgctct tgcgggaagc ccgaaagacg ctgatgacac atcaatctac 3301 atgttttatc aaaaggtcgg cgacaactca atcgacagct ggaaaaacgc gggccgtgtc 3361 tttaaagaca gcgataagtt cgacgccaac gatccgatcc tgaaagatca gacgcaagaa 3421 tggtccggtt ctgcaacctt tacatctgac ggaaaaatcc gtttattcta cactgactat 3481 tccggtaaac attacggcaa acaaagcctg acaacagcgc aggtaaatgt gtcaaaatct 3541 gatgacacac tcaaaatcaa cggagtggaa gatcacaaaa cgatttttga cggagacgga 3601 aaaacatatc agaacgttca gcagtttatc gatgaaggca attatacatc cggcgacaac 3661 catacgctga gagaccctca ctacgttgaa gacaaaggcc ataaatacct tgtattcgaa 3721 gccaacacgg gaacagaaaa cggataccaa ggcgaagaat ctttatttaa caaagcgtac 3781 tacggcggcg gcacgaactt cttccgtaaa gaaagccaga agcttcagca gagcgctaaa 3841 aaacgcgatg ctgagttagc gaacggcgcc ctcggtatca tagagttaaa taatgattac 3901 acattgaaaa aagtaatgaa gccgctgatc acttcaaaca cggtaactga tgaaatcgag 3961 cgcgcgaatg ttttcaaaat gaacggcaaa tggtacttgt tcactgattc acgcggttca 4021 aaaatgacga tcgatggtat taactcaaac gatatttaca tgcttggtta tgtatcaaac 4081 tctttaaccg gcccttacaa gccgctgaac aaaacagggc ttgtgctgca aatgggtctt 4141 gatccaaacg atgtgacatt cacttactct cacttcgcag tgccgcaagc caaaggcaac 4201 aatgtggtta tcacaagcta catgacaaac agaggcttct tcgaggataa aaaggcaaca 4261 tttgcgccaa gcttcttaat gaacatcaaa ggcaataaaa catccgttgt caaaaacagc 4321 atcctggagc aaggacagct gacagtcaac taataacagc aaaaagaaaa tgccgatact 4381 tcattggcat tttcttttat ttctcaacaa gatggtgaat tgactagtgg gtagatccac 4441 aggacgggtg tggtcgccat gatcgcgtag tcgatagtgg ctccaagtag cgaagcgagc 4501 aggactgggc ggcggccaaa gcggtcggac agtgctccga gaacgggtgc gcatagaaat 4561 tgcatcaacg catatagcgc tagcacgcca tagtgactgg cgatgctgtc ggaatggacg 4621 atatcccgca agaggcccgg cagtaccggc ataaccaagc ctatgcctac agcatccagg 4681 gtgacggtgc cgaggatgac gatgagcgca ttgttagatt tcatacacgg tgcctgactg 4741 cgttagcaat ttaactgtga taaactaccg cattaaagct tatcgatgat aagctgtcaa 4801 acatgagaat tgatccggaa cccttaatat aactccgtat aatggatgct atacgaggtt 4861 attaggtccc tcgactatag ggtcaccgtc gacagcgaca cacttgcatc ggatgcagcc 4921 cggttaacgt gccggcacgg cctgggtaac caggtatttt gtccacataa ccgtgcgcaa 4981 aatgttgtgg ataagcagga cacagcagca atccacagca ggcatacaac cgcacaccga 5041 ggttactccg ttctacaggt tacgacgaca tgtcaatact tgcccttgac aggcattgat 5101 ggaatcgtag tctcacgctg atagtctgat cgacaataca agtgggaccg tggtcccaga 5161 ccgataatca gaccgacaac acgagtggga tcgtggtccc agactaataa tcagaccgac 5221 gatacgagtg ggaccgtggt cccagactaa taatcagacc gacgatacga gtgggaccgt 5281 ggttccagac taataatcag accgacgata cgagtgggac catggtccca gactaataat 5341 cagaccgacg atacgagtgg gaccgtggtc ccagtctgat tatcagaccg acgatacgag 5401 tgggaccgtg gtcccagact aataatcaga ccgacgatac gagtgggacc gtggtcccag 5461 actaataatc agaccgacga tacgagtggg accgtggtcc cagactaata atcagaccga 5521 cgatacgagt gggaccgtgg tcccagtctg attatcagac cgacgataca agtggaacag 5581 tgggcccaga gagaatattc aggccagtta tgctttctgg cctgtaacaa aggacattaa 5641 gtaaagacag ataaacgtag actaaaacgt ggtcgcatca gggtgctggc ttttcaagtt 5701 ccttaagaat ggcctcaatt ttctctatac actcagttgg aacacgagac ctgtccaggt 5761 taagcaccat tttatcgccc ttatacaata ctgtcgctcc aggagcaaac tgatgtcgtg 5821 agcttaaact agttcttgat gcagatgacg ttttaagcac agaagttaaa agagtgataa 5881 cttcttcagc ttcaaatatc accccagctt ttttctgctc atgaaggtta gatgcctgct 5941 gcttaagtaa ttcctcttta tctgtaaagg ctttttgaag tgcatcacct gaccgggcag 6001 atagttcacc ggggtgagaa aaaagagcaa caactgattt aggcaatttg gcggtgttga 6061 tacagcgggt aataatctta cgtgaaatat tttccgcatc agccagcgca gaaatatttc 6121 cagcaaattc attctgcaat cggcttgcat aacgctgacc acgttcataa gcacttgttg 6181 ggcgataatc gttacccaat ctggataatg cagccatctg ctcatcatcc agctcgccaa 6241 ccagaacacg ataatcactt tcggtaagtg cagcagcttt acgacggcga ctcccatcgg 6301 caatttctat gacaccagat actcttcgac cgaacgccgg tgtctgttga ccagtcagta 6361 gaaaagaagg gatgagatca tccagtgcgt cctcagtaag cagctcctgg tcacgttcat 6421 tacctgacca tacccgagag gtcttctcaa cactatcacc ccggagcact tcaagagtaa 6481 acttcacatc ccgaccacat acaggcaaag taatggcatt accgcgagcc attactccta 6541 cgcgcgcaat taacgaatcc accatcgggg cagctggtgt cgataacgaa gtatcttcaa 6601 ccggttgagt attgagcgta tgttttggaa taacaggcgc acgcttcatt atctaatctc 6661 ccagcgtggt ttaatcagac gatcgaaaat ttcattgcag acaggttccc aaatagaaag 6721 agcatttctc caggcaccag ttgaagagcg ttgatcaatg gcctgttcaa aaacagttct 6781 catccggatc tgacctttac caacttcatc cgtttcacgt acaacatttt ttagaaccat 6841 gcttccccag gcatcccgaa tttgctcctc catccacggg gactgagagc cattactatt 6901 gctgtatttg gtaagcaaaa tacgtacatc aggctcgaac cctttaagat caacgttctt 6961 gagcagatca cgaagcatat cgaaaaactg cagtgcggag gtgtagtcaa acaactcagc 7021 aggcgtggga acaatcagca catcagcagc acatacgaca ttaatcgtgc cgatacccag 7081 gttaggcgcg ctgtcaataa ctatgacatc atagtcatga gcaacagttt caatggccag 7141 tcggagcatc aggtgtggat cggtgggcag tttaccttca tcaaatttgc ccattaactc 7201 agtttcaata cggtgcagag ccagacagga aggaataatg tcaagccccg gccagcaagt 7261 gggctttatt gcataagtga catcgtcctt ttccccaaga tagaaaggca ggagagtgtc 7321 ttctgcatga atatgaagat ctggtaccca tccgtgatac attgaggctg ttccctgggg 7381 gtcgttacct tccacgagca aaacacgtag ccccttcaga gccagatcct gagcaagatg 7441 aacagaaact gaggttttgt aaacgccacc tttatgggca gcaaccccga tcaccggtgg 7501 aaatacgtct tcagcacgtc gcaatcgcgt accaaacaca tcacgcatat gattaatttg 7561 ttcaattgta taaccaacac gttgctcaac ccgtcctcga atttccatat ccgggtgcgg 7621 tagtcgccct gctttctcgg catctctgat agcctgagaa gaaaccccaa ctaaatccgc 7681 tgcttcacct attctccagc gccgggttat tttcctcgct tccgggctgt catcattaaa 7741 ctgtgcaatg gcgatagcct tcgtcatttc atgaccagcg tttatgcact ggttaagtgt 7801 ttccatgagt ttcattctga acatccttta atcattgctt tgcgtttttt tattaaatct 7861 tgcaatttac tgcaaagcaa caacaaaatc gcaaagtcat caaaaaaccg caaagttgtt 7921 taaaataaga gcaacactac aaaaggagat aagaagagca catacctcag tcacttatta 7981 tcactagcgc tcgccgcagc cgtgtaaccg agcatagcga gcgaactggc gaggaagcaa 8041 agaagaactg ttctgtcaga tagctcttac gctcagcgca agaagaaata tccaccgtgg 8101 gaaaaactcc aggtagaggt acacacgcgg atagccaatt cagagtaata aactgtgata 8161 atcaaccctc atcaatgatg acgaactaac ccccgatatc aggtcacatg acgaagggaa 8221 agagaaggaa atcaactgtg acaaactgcc ctcaaatttg gcttccttaa aaattacagt 8281 tcaaaaagta tgagaaaatc catgcaggct gaaggaaaca gcaaaactgt gacaaattac 8341 cctcagtagg tcagaacaaa tgtgacgaac caccctcaaa tctgtgacag ataaccctca 8401 gactatcctg tcgtcatgga agtgatatcg cggaaggaaa atacgatatg agtcgtctgg 8461 cggcctttct ttttctcaat gtatgagagg cgcattggag ttctgctgtt gatctcatta 8521 acacagacct gcaggaagcg gcggcggaag tcaggcatac gctggtaact ttgaggcagc 8581 tggtaacgct ctatgatcca gtcgattttc agagagacga tgcctgagcc atccggctta 8641 cgatactgac acagggattc gtataaacgc atggcatacg gattggtgat ttcttttgtt 8701 tcactaagcc gaaactgcgt aaaccggttc tgtaacccga taaagaaggg aatgagatat 8761 gggttgatat gtacactgta aagccctctg gatggactgt gcgcacgttt gataaaccaa 8821 ggaaaagatt catagccttt ttcatcgccg gcatcctctt cagggcgata aaaaaccact 8881 tccttccccg cgaaactctt caatgcctgc cgtatatcct tactggcttc cgcagaggtc 8941 aatccgaata tttcagcata tttagcaaca tggatctcgc agataccgtc atgttcctgt 9001 agggtgccat cagattttct gatctggtca acgaacagat acagcatacg tttttgatcc 9061 cgggagagac tatatgccgc ctcagtgagg tcgtttgact ggacgattcg cgggctattt 9121 ttacgtttct tgtgattgat aaccgctgtt tccgccatga cagatccatg tgaagtgtga 9181 caagttttta gattgtcaca ctaaataaaa aagagtcaat aagcagggat aactttgtga 9241 aaaaacagct tcttctgagg gcaatttgtc acagggttaa gggcaatttg tcacagacag 9301 gactgtcatt tgagggtgat ttgtcacact gaaagggcaa tttgtcacaa caccttctct 9361 agaaccagca tggataaagg cctacaaggc gctctaaaaa agaagatcta aaaactataa 9421 aaaaaataat tataaaaata tccccgtgga taagtggata accccaaggg aagttttttc 9481 aggcatcgtg tgtaagcaga atatataagt gctgttccct ggtgcttcct cgctcactcg 9541 aattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt 9601 tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgacgga 9661 tcgcttgcct gtaacttaca cgcgcctcgt aggcctgaga gcaggaagag caagataaaa 9721 ggtagtattt gttggcgatc cccctagagt cttttacatc ttcggaaaac aaaaactatt 9781 ttttctttaa tttctttttt tactttctat ttttaattta tatatttata ttaaaaaatt 9841 taaattataa ttatttttat agcacgtgat gaaaaggacc ctaagaaacc attattatca 9901 tgacattaac ctataaaaat aggcgtatca cgaggccctt tcgtctcgcg cgtttcggtg 9961 atgacggtga aaacctctga cacatgcagc tcccggagac ggtcacagct tgtctgtaag 10021 cggatgccgg gagcagacaa gcccgtcagg gcgcgtcagc gggtgttggc gggtgtcggg 10081 gctggcttaa ctatgcggca tcagagcaga ttgtactgag agtgcaccat aaattcccgt 10141 tttaagagct tggtgagcgc taggagtcac tgccaggtat cgtttgaaca cggcattagt 10201 cagggaagtc ataacacagt cctttcccgc aattttcttt ttctattact cttggcctcc 10261 tctagtacac tctatatttt tttatgcctc ggtaatgatt ttcatttttt tttttccacc 10321 tagcggatga ctcttttttt ttcttagcga ttggcattat cacataatga attatacatt 10381 atataaagta atgtgatttc ttcgaagaat atactaaaaa atgagcaggc aagataaacg 10441 aaggcaaaga tgacagagca gaaagcccta gtaaagcgta ttacaaatga aaccaagatt 10501 cagattgcga tctctttaaa gggtggtccc ctagcgatag agcactcgat cttcccagaa 10561 aaagaggcag aagcagtagc agaacaggcc acacaatcgc aagtgattaa cgtccacaca 10621 ggtatagggt ttctggacca tatgatacat gctctggcca agcattccgg ctggtcgcta 10681 atcgttgagt gcattggtga cttacacata gacgaccatc acaccactga agactgcggg 10741 attgctctcg gtcaagcttt taaagaggcc ctaggggccg tgcgtggagt aaaaaggttt 10801 ggatcaggat ttgcgccttt ggatgaggca ctttccagag cggtggtaga tctttcgaac 10861 aggccgtacg cagttgtcga acttggtttg caaagggaga aagtaggaga tctctcttgc 10921 gagatgatcc cgcattttct tgaaagcttt gcagaggcta gcagaattac cctccacgtt 10981 gattgtctgc gaggcaagaa tgatcatcac cgtagtgaga gtgcgttcaa ggctcttgcg 11041 gttgccataa gagaagccac ctcgcccaat ggtaccaacg atgttccctc caccaaaggt 11101 gttcttatgt agtgacaccg attatttaaa gctgcagcat acgatatata tacatgtgta 11161 tatatgtata cctatgaatg tcagtaagta tgtatacgaa cagtatgata ctgaagatga 11221 caaggtaatg catcattcta tacgtgtcat tctgaacgag gcgcgctttc cttttttctt 11281 tttgcttttt cttttttttt ctcttgaact cgacggatct atgcggtgtg aaataccgca 11341 cagatgcgta aggagaaaat accgcatcag gaaattgtaa gcgttaattc gagggcttcg 11401 ccctgtcgct caactgcggc gagcactact ggctgtaaaa ggacagacca catcatggtt 11461 ctgtgttcat taggttgttc tgtccattgc tgacataatc cgctccactt caacgtaaca 11521 ccgcacgaag atttctattg ttcctgaagg catattcaaa tcgttttcgt taccgcttgc 11581 aggcatcatg acagaacact acttcctata aacgctacac aggctcctga gattaataat 11641 gcggatctct acgataatgg gagatttttc cgactgtttc gttcgcttct cagtggataa 11701 cagccagctt ctctgtttaa cagacaaaaa cagcatatcc actcagttcc acatttccat 11761 ataaaggcca aggcatttat tctcaggata attgtttcag catcgcaacc gcatcagact 11821 ccggcatcgc aaactgcacc cggtgccggg cagccacatc cagcgcaaaa accttcgtgt 11881 agacttccgt tgaactgatg gacttatgtc ccatcaggct ttgcagaact ttcagcggta 11941 taccggcata cagcatgtgc atcgcatagg aatggcggaa cgtatgtggt gtgaccggaa 12001 cagagaacgt cacaccgtca gcagcagcgg cggcaaccgc ctccccaatc caggtcctga 12061 ccgttctgtc cgtcacttcc cagatccgcg ctttctctgt ccttcctgtg cgacggttac 12121 gccgctccat gagcttatcg cgaataaata cctgtgacgg aagatcactt cgcagaataa 12181 ataaatcctg gtgtccctgt tgataccggg aagccctggg ccaacttttg gcgaaaatga 12241 gacgttgatc ggcacgtaag aggttccaac tttcaccata atgaaataag atcactaccg 12301 ggcgtatttt ttgagttatc gagattttca ggagctaagg aagctaaaat ggagaaaaaa 12361 atcactggat ataccaccgt tgatatatcc caatggcatc gtaaagaaca ttttgaggca 12421 tttcagtcag ttgctcaatg tacctataac cagaccgttc agctggatat tacggccttt 12481 ttaaagaccg taaagaaaaa taagcacaag ttttatccgg cctttattca cattcttgcc 12541 cgcctgatga atgctcatcc ggagttccgt atggcaatga aagacggtga gctggtgata 12601 tgggatagtg ttcacccttg ttacaccgtt ttccatgagc aaactgaaac gttttcatcg 12661 ctctggagtg aataccacga cgatttccgg cagtttctac acatatattc gcaagatgtg 12721 gcgtgttacg gtgaaaacct ggcctatttc cctaaagggt ttattgagaa tatgtttttc 12781 gtctcagcca atccctgggt gagtttcacc agttttgatt taaacgtggc caatatggac 12841 aacttcttcg cccccgtttt caccatgggc aaatattata cgcaaggcga caaggtgctg 12901 atgccgctgg cgattcaggt tcatcatgcc gtttgtgatg gcttccatgt cggcagaatg 12961 cttaatgaat tacaacagta ctgcgatgag tggcagggcg gggcgtaatt tttttaaggc 13021 agttattggt gcccttaaac gcctggttgc tacgcctgaa taagtgataa taagcggatg 13081 aatggcagaa attcgatgat aagctgtcaa acatgagaat tggtcgacgg cgcgccaaag 13141 cttgcatgcc tgcagccgcg taacctggca aaatcggtta cggttgagta ataaatggat 13201 gccctgcgta agcggggcac atttcattac ctctttctcc gcacccgaca tagataataa 13261 cttcgtatag tatacattat acgaagttat ctagtagact taattaagga tcgatccggc 13321 gcgccaatag tcatgccccg cgcccaccgg aaggagctga ctgggttgaa ggctctcaag 13381 ggcatcggtc gagcttgaca ttgtaggact atattgctct aataaatttg cggccgctaa 13441 tacgactcac tatagggaga ggatcg //