ƚV `$  j---$$I_K+Q]le9"Lys_Q F@Au@DMZFlw)N+pWO S} ]1:Tt\)X#`g4E  ou=c VMIITW_/k`{5 W2u\MM~rS1) MmZ)[(g;nM*{~iN[QLLPNYfx\2 6a{k!_UWTT--I@TL-TI--*02Arialw@ ww w0- . 2 WPPAC4."System &$w-  02Arialw@ ww w0- . 2 W19500 bp.- -f--"$-n@v-RGoq- -v90 GRe\GR-v----*02Arialw@ ww w0- b. 2 d SACBII gene.-- -f--$ GzusdzxjA7- s90 7e\7xzds---*02Arialw@ ww w0- \. 2  KANAMYCINn.-- -f--$- 90 e\---*02Arialw@ ww w0- .2 BLS (Blasticidin-S-Methylase).-- ---.$N<DQc~yO"6`~qKiRh- <N90 hRe\hR<N---*02Arialw@ ww w0- {dw. 2 {ORIP.-- -%R(Z{--*02Arialw@ ww w0- "(R. 2 LLOXP511p.-- -%`'fz--*02Arialw@ ww w0- ,'`. 2 TN7ATT.-- -%w6C--*02Arialw@ ww w0-  >w. 2 6@SP6A.-- -%5B--*02Arialw@ ww w0- %5. 2 O PBR322-SEQ.-- ---$X~z{^gcj~- ^{ cg~jsjcg^~j~Xz{---*02Arialw@ ww w0- ~{.2 ~ SV40-PROMOTER.-- ---$ sZ%!Qz- 90 ze\zs---*02Arialw@ ww w0- mrT. 2 t SV40-POLYA.-- ---$^mm- 90 me\m^m---*02Arialw@ ww w0- (2. 2 R4 LAMBDA cos.-- ---$ FPID=h~Z;$- ~=D90 Z$e\Z~$FIP=D---*02Arialw@ ww w0- )V.2  HCMV-Promoter.-- -%2j,--*02Arialw@ ww w0- j2. 2 LOXG.-- -%%x--*02Arialw@ ww w0- 4T%.2 4*T7.-- ---$ "QT_ y- _ ysjy"QT_---*02Arialw@ ww w0- 6. 2 6wTN903T.-- ---2$`_* 6i: Gq )HT- 6  TsjT`_*6 ---*02Arialw@ ww w0- Lq. 2 LPUC-LINK.-- -%Y'`z--*02Arialw@ ww w0- 'Y. 2 pI-SCEI.-- - ~80&~--*02Arialw@ ww w0- . 2 E.COLI.-- -ff--z$;'I{Bp ,R4|Pi}7i0_t]C$ |4SI(ZgosrrDmdVD.rN.d9 V)#-   #sj #'---*02Arialw@ ww w0- Ho.2 <~P1 Plasmid Replicon.-- -%"-- 0Times New Romanww w0- 6. 2  AscI (16978).-- -%q&uy-- 0Times New Romanww w0- 8&q. 2  AscI (19354).-- -%{:G-- 0Times New Romanww w0- @U{. 2 `V NotI (2778)).-- -%%x-- 0Times New Romanww w0- ]T%. 2 ] NotI (19466).-- -%%x-- 0Times New Romanww w0- %. 2  BamHI (1)6.-- -%v5B-- 0Times New Romanww w0- *v. 2 + BamHI (2739).-- -%%x-- 0Times New Romanww w0- o%. 2 p ScaI (21)3.-- -%-- 0Times New Romanww w0- v. 2 v ScaI (1789)).-- -%t 4@-- 0Times New Romanww w0-   t. 2  ScaI (2723)).-- -%p0<-- 0Times New Romanww w0- p. 2  EcoRI (2692).-- -%r-- 0Times New Romanww w0- N. 2 O EcoRI (6327).-- -%k-- 0Times New Romanww w0- F. 2 f EcoRI (8439).-- -%-- 0Times New Romanww w0- u.2  EcoRI (10623).-- -%%y-- 0Times New Romanww w0- I%. 2 J HindIII (57).-- -%-- 0Times New Romanww w0- u.2 vHindIII (3748).-- -%&-- 0Times New Romanww w0- .2 HindIII (4197).-- -%-- 0Times New Romanww w0- .2 HindIII (4851).-- -%-- 0Times New Romanww w0- .2 4HindIII (5332).-- -%,o0-- 0Times New Romanww w0- o,.2 HindIII (7498).-- -%,9-- 0Times New Romanww w0- ,.2 'HindIII (10229).-- -%Bnd-- 0Times New Romanww w0- FB.2 fHindIII (11476).-- -%il-- 0Times New Romanww w0- `li.2 ` HindIII (16171).-- -%p&ty-- 0Times New Romanww w0- &p.2 HindIII (19346).--