LOCUS Exported 18525 bp ds-DNA circular SYN 01-MAR-2014 DEFINITION . ACCESSION . VERSION . KEYWORDS pBAC-red SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 18525) AUTHORS . TITLE Direct Submission JOURNAL Exported Saturday, Mar 01, 2014 from SnapGene 2.2.2 http://www.snapgene.com COMMENT cI-857 (temperature sensitive lambda repressor) controls expression of the lambda recombination gene exo-bet-gam. The entire segment was inserted as a 7,205 bp I-SceI fragment into pBACe3.6 by Michael Nefedov.  FEATURES Location/Qualifiers source 1..18525 /organism="synthetic DNA construct" /lab_host="Escherichia coli" /mol_type="other DNA" misc_feature 1..2810 /note="pUC-Link" misc_feature 2817..2834 /note="SP6" misc_feature 2855..2879 /note="attB2" CDS 2934..4352 /note="SacB" misc_recomb 4625..11849 /note="CI-exo-bet-gam" /note="Lambda CI-exo-bet-gam cassette" misc_signal 4625..4642 /note="I-SceI" promoter 4834..5547 /note="cI-857(ts)" CDS 9542..9958 /note="gam" CDS 9964..10749 /note="bet" CDS 10746..11426 /note="exo" CDS 10746..11426 /codon_start=1 /note="exo" /translation="MTPDIILQRTGIDVRAVEQGDDAWHKLRLGVITASEVHNVIAKPR SGKKWPDMKMSYFHTLLAEVCTGVAPEVNAKALAWGKQYENDARTLFKFTSGVNVTESP IIYRDESMRTACSPDGLCSDGNGLELKCPFTSRDFMKFRLGGFEAIKSAYMAQVQYSMW VTRKNAWYFANYDPRMKREGLHYVVIERDEKYMASFDEIVPEFIEKMDEALAEIGFVFG EQWR" misc_feature 11830..11847 /note="I-SceI" misc_feature 12012..12529 /note="parC" CDS complement(12602..13573) /note="parB" CDS complement(13573..14748) /note="parA" CDS complement(15327..16082) /note="repE" rep_origin 16421..16476 /note="oriS" misc_feature complement(16856..17203) /note="redF" CDS 17422..18081 /note="CM(R)" misc_feature 18293..18329 /note="PI-SceI" misc_feature 18330..18354 /note="attB1" promoter 18453..18493 /note="SacB gene promoter" misc_feature 18502..18524 /note="T7" ORIGIN 1 gatccgcgga attcgagctc acgcgtactg atgcatgatc cgggtttaaa cccagtactc 61 tagatcctct agagtcgacc tgcaggcatg caagcttggc gtaatcatgg tcatagctgt 121 ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa catacgagcc ggaagcataa 181 agtgtaaagc ctggggtgcc taatgagtga gctaactcac attaattgcg ttgcgctcac 241 tgcccgcttt ccagtcggga aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg 301 cggggagagg cggtttgcgt attgggcgct cttccgcttc ctcgctcact gactcgctgc 361 gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat 421 ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca 481 ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc 541 atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc 601 aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg 661 gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcaatgc tcacgctgta 721 ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg 781 ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac 841 acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag 901 gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga aggacagtat 961 ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat 1021 ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc 1081 gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt 1141 ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct 1201 agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt 1261 ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc 1321 gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac 1381 catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat 1441 cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg 1501 cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata 1561 gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta 1621 tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt 1681 gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag 1741 tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa 1801 gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc 1861 gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt 1921 taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc 1981 tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta 2041 ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa 2101 taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca 2161 tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac 2221 aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgacgtctaa gaaaccatta 2281 ttatcatgac attaacctat aaaaataggc gtatcacgag gccctttcgt ctcgcgcgtt 2341 tcggtgatga cggtgaaaac ctctgacaca tgcagctccc ggagacggtc acagcttgtc 2401 tgtaagcgga tgccgggagc agacaagccc gtcagggcgc gtcagcgggt gttggcgggt 2461 gtcggggctg gcttaactat gcggcatcag agcagattgt actgagagtg caccatatgc 2521 ggtgtgaaat accgcacaga tgcgtaagga gaaaataccg catcaggcgc cattcgccat 2581 tcaggctgcg caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc 2641 tggcgaaagg gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt 2701 cacgacgttg taaaacgacg gccagtgaat tcgagctcgg tacccgggga tctagagtac 2761 tggttgaaac ccggatcatg catcagtacg cgtgagctcg aattccgcgg atccttctat 2821 agtgtcacct aaatgtcgac ggccaggcgg ccgcacccag ctttcttgta caaagtggtg 2881 aggatcgaaa cggcagatcg caaaaaacag tacatacaga aggagacatg aacatgaaca 2941 tcaaaaaaat tgtaaaacaa gccacagttc tgacttttac gactgcactt ctggcaggag 3001 gagcgactca agccttcgcg aaagaaaata accaaaaagc atacaaagaa acgtacggcg 3061 tctctcatat tacacgccat gatatgctgc agatccctaa acagcagcaa aacgaaaaat 3121 accaagtgcc tcaattcgat caatcaacga ttaaaaatat tgagtctgca aaaggacttg 3181 atgtgtggga cagctggccg ctgcaaaacg ctgacggaac agtagctgaa tacaacggct 3241 atcacgttgt gtttgctctt gcgggaagcc cgaaagacgc tgatgacaca tcaatctaca 3301 tgttttatca aaaggtcggc gacaactcaa tcgacagctg gaaaaacgcg ggccgtgtct 3361 ttaaagacag cgataagttc gacgccaacg atccgatcct gaaagatcag acgcaagaat 3421 ggtccggttc tgcaaccttt acatctgacg gaaaaatccg tttattctac actgactatt 3481 ccggtaaaca ttacggcaaa caaagcctga caacagcgca ggtaaatgtg tcaaaatctg 3541 atgacacact caaaatcaac ggagtggaag atcacaaaac gatttttgac ggagacggaa 3601 aaacatatca gaacgttcag cagtttatcg atgaaggcaa ttatacatcc ggcgacaacc 3661 atacgctgag agaccctcac tacgttgaag acaaaggcca taaatacctt gtattcgaag 3721 ccaacacggg aacagaaaac ggataccaag gcgaagaatc tttatttaac aaagcgtact 3781 acggcggcgg cacgaacttc ttccgtaaag aaagccagaa gcttcagcag agcgctaaaa 3841 aacgcgatgc tgagttagcg aacggcgccc tcggtatcat agagttaaat aatgattaca 3901 cattgaaaaa agtaatgaag ccgctgatca cttcaaacac ggtaactgat gaaatcgagc 3961 gcgcgaatgt tttcaaaatg aacggcaaat ggtacttgtt cactgattca cgcggttcaa 4021 aaatgacgat cgatggtatt aactcaaacg atatttacat gcttggttat gtatcaaact 4081 ctttaaccgg cccttacaag ccgctgaaca aaacagggct tgtgctgcaa atgggtcttg 4141 atccaaacga tgtgacattc acttactctc acttcgcagt gccgcaagcc aaaggcaaca 4201 atgtggttat cacaagctac atgacaaaca gaggcttctt cgaggataaa aaggcaacat 4261 ttgcgccaag cttcttaatg aacatcaaag gcaataaaac atccgttgtc aaaaacagca 4321 tcctggagca aggacagctg acagtcaact aataacagca aaaagaaaat gccgatactt 4381 cattggcatt ttcttttatt tctcaacaag atggtgaatt gactagtggg tagatccaca 4441 ggacgggtgt ggtcgccatg atcgcgtagt cgatagtggc tccaagtagc gaagcgagca 4501 ggactgggcg gcggccaaag cggtcggaca gtgctccgag aacgggtgcg catagaaatt 4561 gcatcaacgc atatagcgct agctcggaat ggacgatatc ccgcgacagc gacacacttg 4621 cgttattacc ctgttatccc tatgatcgcg ctttgatata cgccgagatc tttagctgtc 4681 ttggtttgcc caaagcgcat tgcataatct ttcagggtta tgcgttgttc catacaacct 4741 ccttagtaca tgcaaccatt atcaccgcca gaggtaaaat agtcaacacg cacggtgtta 4801 gatatttatc ccttgcggtg atagatttaa cgtatgagca caaaaaagaa accattaaca 4861 caagagcagc ttgaggacgc acgtcgcctt aaagcaattt atgaaaaaaa gaaaaatgaa 4921 cttggcttat cccaggaatc tgtcgcagac aagatgggga tggggcagtc aggcgttggt 4981 gctttattta atggcatcaa tgcattaaat gcttataacg ccgcattgct tgcaaaaatt 5041 ctcaaagtta gcgttgaaga atttagccct tcaatcgcca gagaaatcta cgagatgtat 5101 gaagcggtta gtatgcagcc gtcacttaga agtgagtatg agtaccctgt tttttctcat 5161 gttcaggcag ggatgttctc acctgagctt agaaccttta ccaaaggtga tgcggagaga 5221 tgggtaagca caaccaaaaa agccagtgat tctgcattct ggcttgaggt tgaaggtaat 5281 tccatgaccg caccaacagg ctccaagcca agctttcctg acggaatgtt aattctcgtt 5341 gaccctgagc aggctgttga gccaggtgat ttctgcatag ccagacttgg gggtgatgag 5401 tttaccttca agaaactgat cagggatagc ggtcaggtgt ttttacaacc actaaaccca 5461 cagtacccaa tgatcccatg caatgagagt tgttccgttg tggggaaagt tatcgctagt 5521 cagtggcctg aagagacgtt tggctgatcg gcaaggtgtt ctggtcggcg catagctgat 5581 aacaattgag caagaatctt catcgaatta ggggaatttt cactcccctc agaacataac 5641 atagtaaatg gattgaatta tgaagaatgg tttttatgcg acttaccgca gcaaaaataa 5701 agggaaagat aagcgctcaa taaacctgtc tgttttcctt aattctctgc tggctgataa 5761 tcatcacctg caggttggct ccaattattt gtatattcat aaaatcgatg gaaaaacttt 5821 tctctttacc aaaacaaatg acaagagtct ggttcagaag ataaatcgct ctaaagcttc 5881 agttgaagat attaagaaca gcctcgcaga tgacgaatca ttgggattcc catctttttt 5941 gtttgttgaa ggcgacacca ttggttttgc cagaactgtt ttcgggccga ccacatccga 6001 tctgacagat tttttaatcg ggaaaggaat gtcattaagc agtggagagc gcgttcagat 6061 agagccactg atgaggggaa ccaccaaaga cgatgttatg catatgcatt tcatcggccg 6121 aacaacggtg aaggtagaag ccaagctacc tgtatttggc gatatattaa aggtcttagg 6181 ggcaacagat attgaagggg agctttttga ctcattggat atagtcatta agccaaaatt 6241 taaaagggat ataaaaaagg ttgccaagga tattattttt aacccgtcac ctcaattttc 6301 agacattagc ctgcgggcaa aagatgaggc cggagatatt ttaacagaac attatctatc 6361 agaaaaaggc catctctcag cgcctctgaa caaggtcacc aatgctgaga tagctgaaga 6421 gatggcatat tgctacgcaa gaatgaaaag tgatatactg gaatgtttta aaaggcaggt 6481 gggcaaagtt aaggattaat tatcaggagt aattatgcgg aacagaatca tgcctggtgt 6541 ttacatagta ataattcctt acgttatcgt aagcatttgc tatctccttt tccgccacta 6601 cattcctggt gtttcttttt cagctcatag agatggtctt ggggcgacat tgtcatcata 6661 tgcaggaacc atgattgcaa tcctgattgc tgccttgacg tttctaatcg gaagcagaac 6721 gcgccgactg gccaagatta gagagtatgg gtatatgaca tcggtagtta ttgtctatgc 6781 ccttagtttt gttgagcttg gagctttgtt tttctgcggg ttattgcttc tttccagcat 6841 aagcggctac atgataccca ctatcgccat cggcattgcc tctgcatcgt tcattcatat 6901 atgcatcctt gttttccaac tatataattt gaccagagaa caagaataac ccggcctcag 6961 cgccgggttt tctttgcctc acgatcgccc ccaaaacaca taaccaattg tatttattga 7021 aaaataaata gatacaactc actaaacata gcaattcaga tctctcacct accaaacaat 7081 gcccccctgc aaaaaataaa ttcatataaa aaacatacag ataaccatct gcggtgataa 7141 attatctctg gcggtgttga cataaatacc actggcggtg atactgagca catcagcagg 7201 acgcactgac caccatgaag gtgacgctct taaaaattaa gccctgaaga agggcagcat 7261 tcaaagcaga aggctttggg gtgtgtgata cgaaacgaag cattggccgt aagtgcgatt 7321 ccggattagc tgccaatgtg ccaatcgcgg ggggttttcg ttcaggacta caactgccac 7381 acaccaccaa agctaactga caggagaatc cagatggatg cacaaacacg ccgccgcgaa 7441 cgtcgcgcag agaaacaggc tcaatggaaa gcagcaaatc ccctgttggt tggggtaagc 7501 gcaaaaccag ttaaccgccc tattctctcg ctgaatcgca aaccgaaatc acgagtagaa 7561 agcgcactaa atccgataga ccttacagtg ctggctgaat accacaaaca gattgaaagc 7621 aacctgcaac gtattgagcg caagaatcag cgcacatggt acagcaagcc tggcgaacgc 7681 ggcataacat gcagtggacg ccagaaaatt aagggaaaat cgattcctct tatctagtta 7741 cttagatatt ggccttggct ttatctcaat attatatgga tcatagctgg caactaattc 7801 agtccagtaa atatcctcaa tagggaataa tatatgcttt ccattccatc gggaaaaagt 7861 tttgttcaac acaccaagct caatcaactc actaatgtat gggaattgtt ttgatgtaac 7921 cacatacttc ctgccttcat taagggctgc gcacaaaacc atagattgct cttctgtaag 7981 gttttgaatt actgatcgca ctttatcgtt ttgcatctta atgcgttttc ttagcttaaa 8041 tcgcttatat ctggcgctgg caatagctga taatcgatgc acattaattg ctagcgaaaa 8101 tgcaagagca aagacgaaaa catgccacac atgaggaata ccgattctct cattaacata 8161 ttcaggccag ttatctgggc ttaaaagcag aagtccaacc cagataacga tcatatacat 8221 ggttctctcc agaggttcat tactgaacac tcgtccgaga ataacgagtg gatccatttc 8281 tatactcatc aaactgtagg ggttgtaata gtttatccga tttctcgctg taggggtaca 8341 cgagaaccac cgagcctgat gtggttaaaa gacaggcaca atctttacta ccgcaatcca 8401 ctatttaagg tgatatatgg aagaagaatt tgaagagttc gaagagcatc ctcaggatgt 8461 gatggaacaa taccaggact atccgtatga ctacgactat tgataaaaat caatggtgtg 8521 gacaattcaa gcgatgcaat ggatgcaagc tgcaatcgga atgcatggtt aagcctgaag 8581 aaatgtttcc tgtaatggaa gatgggaaat atgtcgataa atgggcaata cgaacgacgg 8641 caatgattgc cagagaactt ggtaaacaga acaacaaagc tgcctgatag tggcctttat 8701 ttttggcata aataacagaa taaacactgc actgtgtatt cattccaacg agtgaataca 8761 cggagcaatg tcgctcgtaa ctaaacagga gccgacttgt tctgattatt ggaaatcttc 8821 tttgccctcc agtgtgaggg cgatttttta tctgtgagga tatgaacaga tgtcaaacat 8881 caaaaaatac atcattgatt acgactggaa agcatcaata gaaattgaaa tcgaccatga 8941 cgtaatgaca gaggaaaaac ttcaccagat taataatttc tggtcagact ctgaataccg 9001 actcaataaa cacggctctg tattaaatgc tgtattaatc atgctggcgc aacatgctct 9061 gcttatagca atttcaagcg acttaaatgc atatggtgtt gtgtgtgagt tcgactggaa 9121 tgatggaaat ggtcaggaag gatggcctcc aatggatggt agcgaaggaa taagaattac 9181 cgatatcgat acatcaggaa tatttgattc agatgatatg actatcaagg ccgcctgagt 9241 gcggttttac cgcataccaa taacgcttca ctcgaggcgt ttttcgttat gtataaataa 9301 ggagcacacc atgcaatatg ccattgcagg gtggcctgtt gctggctgcc cttccgaatc 9361 tttacttgaa cgaatcaccc gtaaattacg tgacggatgg aaacgcctta tcgacatact 9421 taatcagcca ggagtcccaa agaatggatc aaacacttat ggctatccag actaaattca 9481 ctatcgccac ttttattggc gatgaaaaga tgtttcgtga agccgtcgac gcttataaaa 9541 aatggatatt aatactgaaa ctgagatcaa gcaaaagcat tcactaaccc cctttcctgt 9601 tttcctaatc agcccggcat ttcgcgggcg atattttcac agctatttca ggagttcagc 9661 catgaacgct tattacattc aggatcgtct tgaggctcag agctgggcgc gtcactacca 9721 gcagctcgcc cgtgaagaga aagaggcaga actggcagac gacatggaaa aaggcctgcc 9781 ccagcacctg tttgaatcgc tatgcatcga tcatttgcaa cgccacgggg ccagcaaaaa 9841 atccattacc cgtgcgtttg atgacgatgt tgagtttcag gagcgcatgg cagaacacat 9901 ccggtacatg gttgaaacca ttgctcacca ccaggttgat attgattcag aggtataaaa 9961 cgaatgagta ctgcactcgc aacgctggct gggaagctgg ctgaacgtgt cggcatggat 10021 tctgtcgacc cacaggaact gatcaccact cttcgccaga cggcatttaa aggtgatgcc 10081 agcgatgcgc agttcatcgc attactgatc gttgccaacc agtacggcct taatccgtgg 10141 acgaaagaaa tttacgcctt tcctgataag cagaatggca tcgttccggt ggtgggcgtt 10201 gatggctggt cccgcatcat caatgaaaac cagcagtttg atggcatgga ctttgagcag 10261 gacaatgaat cctgtacatg ccggatttac cgcaaggacc gtaatcatcc gatctgcgtt 10321 accgaatgga tggatgaatg ccgccgcgaa ccattcaaaa ctcgcgaagg cagagaaatc 10381 acggggccgt ggcagtcgca tcccaaacgg atgttacgtc ataaagccat gattcagtgt 10441 gcccgtctgg ccttcggatt tgctggtatc tatgacaagg atgaagccga gcgcattgtc 10501 gaaaatactg catacactgc agaacgtcag ccggaacgcg acatcactcc ggttaacgat 10561 gaaaccatgc aggagattaa cactctgctg atcgccctgg ataaaacatg ggatgacgac 10621 ttattgccgc tctgttccca gatatttcgc cgcgacattc gtgcatcgtc agaactgaca 10681 caggccgaag cagtaaaagc tcttggattc ctgaaacaga aagccgcaga gcagaaggtg 10741 gcagcatgac accggacatt atcctgcagc gtaccgggat cgatgtgaga gctgtcgaac 10801 agggggatga tgcgtggcac aaattacggc tcggcgtcat caccgcttca gaagttcaca 10861 acgtgatagc aaaaccccgc tccggaaaga agtggcctga catgaaaatg tcctacttcc 10921 acaccctgct tgctgaggtt tgcaccggtg tggctccgga agttaacgct aaagcactgg 10981 cctggggaaa acagtacgag aacgacgcca gaaccctgtt taaattcact tccggcgtga 11041 atgttactga atccccgatc atctatcgcg acgaaagtat gcgtaccgcc tgctctcccg 11101 atggtttatg cagtgacggc aacggccttg aactgaaatg cccgtttacc tcccgggatt 11161 tcatgaagtt ccggctcggt ggtttcgagg ccataaagtc agcttacatg gcccaggtgc 11221 agtacagcat gtgggtgacg cgaaaaaatg cctggtactt tgccaactat gacccgcgta 11281 tgaagcgtga aggcctgcat tatgtcgtga ttgagcggga tgaaaagtac atggcgagtt 11341 ttgacgagat cgtgccggag ttcatcgaaa aaatggacga ggcactggct gaaattggtt 11401 ttgtatttgg ggagcaatgg cgatgacgca tcctcacgat aatatccggg taggcgcaat 11461 cactttcgtc tactccgtta caaagcgagg ctgggtattt cccggccttt ctgttatccg 11521 aaatccactg aaagcacagc ggctggctga ggagataaat aataaacgag gggctgtatg 11581 cacaaagcat cttctgttga gttaagaacg agtatcgaga tggcacatag ccttgctcaa 11641 attggaatca ggtttgtgcc aataccagta gaaacagacg aagaatttca tacgttagcc 11701 gcatcccttt cacaaaagct ggaaatgatg gtggcgaaag cagaagcaga tgagagaaac 11761 caggtatgac aaccacggaa tgcatttttc tggcagcggg cttcatattc tgtgtgctta 11821 tgcttgccga ttaccctgtt atccctagga acgtgccggc acggcctggg taaccaggta 11881 ttttgtccac ataaccgtgc gcaaaatgtt gtggataagc aggacacagc agcaatccac 11941 agcaggcata caaccgcaca ccgaggttac tccgttctac aggttacgac gacatgtcaa 12001 tacttgccct tgacaggcat tgatggaatc gtagtctcac gctgatagtc tgatcgacaa 12061 tacaagtggg accgtggtcc cagaccgata atcagaccga caacacgagt gggatcgtgg 12121 tcccagacta ataatcagac cgacgatacg agtgggaccg tggtcccaga ctaataatca 12181 gaccgacgat acgagtggga ccgtggttcc agactaataa tcagaccgac gatacgagtg 12241 ggaccgtggt cccagactaa taatcagacc gacgatacga gtgggaccat ggtcccagac 12301 taataatcag accgacgata cgagtgggac cgtggtccca gtctgattat cagaccgacg 12361 atacgagtgg gaccgtggtc ccagactaat aatcagaccg acgatacgag tgggaccgtg 12421 gtcccagact aataatcaga ccgacgatac gagtgggacc gtggtcccag tctgattatc 12481 agaccgacga tacaagtgga acagtgggcc cagagagaat attcaggcca gttatgcttt 12541 ctggcctgta acaaaggaca ttaagtaaag acagataaac gtagactaaa acgtggtcgc 12601 atcagggtgc tggcttttca agttccttaa gaatggcctc aattttctct atacactcag 12661 ttggaacacg agacctgtcc aggttaagca ccattttatc gcccttatac aatactgtcg 12721 ctccaggagc aaactgatgt cgtgagctta aactagttct tgatgcagat gacgttttaa 12781 gcacagaagt taaaagagtg ataacttctt cagcttcaaa tatcacccca gcttttttct 12841 gctcatgaag gttagatgcc tgctgcttaa gtaattcctc tttatctgta aaggcttttt 12901 gaagtgcatc acctgaccgg gcagatagtt caccggggtg agaaaaaaga gcaacaactg 12961 atttaggcaa tttggcggtg ttgatacagc gggtaataat cttacgtgaa atattttccg 13021 catcagccag cgcagaaata tttccagcaa attcattctg caatcggctt gcataacgct 13081 gaccacgttc ataagcactt gttgggcgat aatcgttacc caatctggat aatgcagcca 13141 tctgctcatc atccagctcg ccaaccagaa cacgataatc actttcggta agtgcagcag 13201 ctttacgacg gcgactccca tcggcaattt ctatgacacc agatactctt cgaccgaacg 13261 ccggtgtctg ttgaccagtc agtagaaaag aagggatgag atcatccagt gcgtcctcag 13321 taagcagctc ctggtcacgt tcattacctg accatacccg agaggtcttc tcaacactat 13381 caccccggag cacttcaaga gtaaacttca catcccgacc acatacaggc aaagtaatgg 13441 cattaccgcg agccattact cctacgcgcg caattaacga atccaccatc ggggcagctg 13501 gtgtcgataa cgaagtatct tcaaccggtt gagtattgag cgtatgtttt ggaataacag 13561 gcgcacgctt cattatctaa tctcccagcg tggtttaatc agacgatcga aaatttcatt 13621 gcagacaggt tcccaaatag aaagagcatt tctccaggca ccagttgaag agcgttgatc 13681 aatggcctgt tcaaaaacag ttctcatccg gatctgacct ttaccaactt catccgtttc 13741 acgtacaaca ttttttagaa ccatgcttcc ccaggcatcc cgaatttgct cctccatcca 13801 cggggactga gagccattac tattgctgta tttggtaagc aaaatacgta catcaggctc 13861 gaacccttta agatcaacgt tcttgagcag atcacgaagc atatcgaaaa actgcagtgc 13921 ggaggtgtag tcaaacaact cagcaggcgt gggaacaatc agcacatcag cagcacatac 13981 gacattaatc gtgccgatac ccaggttagg cgcgctgtca ataactatga catcatagtc 14041 atgagcaaca gtttcaatgg ccagtcggag catcaggtgt ggatcggtgg gcagtttacc 14101 ttcatcaaat ttgcccatta actcagtttc aatacggtgc agagccagac aggaaggaat 14161 aatgtcaagc cccggccagc aagtgggctt tattgcataa gtgacatcgt ccttttcccc 14221 aagatagaaa ggcaggagag tgtcttctgc atgaatatga agatctggta cccatccgtg 14281 atacattgag gctgttccct gggggtcgtt accttccacg agcaaaacac gtagcccctt 14341 cagagccaga tcctgagcaa gatgaacaga aactgaggtt ttgtaaacgc cacctttatg 14401 ggcagcaacc ccgatcaccg gtggaaatac gtcttcagca cgtcgcaatc gcgtaccaaa 14461 cacatcacgc atatgattaa tttgttcaat tgtataacca acacgttgct caacccgtcc 14521 tcgaatttcc atatccgggt gcggtagtcg ccctgctttc tcggcatctc tgatagcctg 14581 agaagaaacc ccaactaaat ccgctgcttc acctattctc cagcgccggg ttattttcct 14641 cgcttccggg ctgtcatcat taaactgtgc aatggcgata gccttcgtca tttcatgacc 14701 agcgtttatg cactggttaa gtgtttccat gagtttcatt ctgaacatcc tttaatcatt 14761 gctttgcgtt tttttattaa atcttgcaat ttactgcaaa gcaacaacaa aatcgcaaag 14821 tcatcaaaaa accgcaaagt tgtttaaaat aagagcaaca ctacaaaagg agataagaag 14881 agcacatacc tcagtcactt attatcacta gcgctcgccg cagccgtgta accgagcata 14941 gcgagcgaac tggcgaggaa gcaaagaaga actgttctgt cagatagctc ttacgctcag 15001 cgcaagaaga aatatccacc gtgggaaaaa ctccaggtag aggtacacac gcggatagcc 15061 aattcagagt aataaactgt gataatcaac cctcatcaat gatgacgaac taacccccga 15121 tatcaggtca catgacgaag ggaaagagaa ggaaatcaac tgtgacaaac tgccctcaaa 15181 tttggcttcc ttaaaaatta cagttcaaaa agtatgagaa aatccatgca ggctgaagga 15241 aacagcaaaa ctgtgacaaa ttaccctcag taggtcagaa caaatgtgac gaaccaccct 15301 caaatctgtg acagataacc ctcagactat cctgtcgtca tggaagtgat atcgcggaag 15361 gaaaatacga tatgagtcgt ctggcggcct ttctttttct caatgtatga gaggcgcatt 15421 ggagttctgc tgttgatctc attaacacag acctgcagga agcggcggcg gaagtcaggc 15481 atacgctggt aactttgagg cagctggtaa cgctctatga tccagtcgat tttcagagag 15541 acgatgcctg agccatccgg cttacgatac tgacacaggg attcgtataa acgcatggca 15601 tacggattgg tgatttcttt tgtttcacta agccgaaact gcgtaaaccg gttctgtaac 15661 ccgataaaga agggaatgag atatgggttg atatgtacac tgtaaagccc tctggatgga 15721 ctgtgcgcac gtttgataaa ccaaggaaaa gattcatagc ctttttcatc gccggcatcc 15781 tcttcagggc gataaaaaac cacttccttc cccgcgaaac tcttcaatgc ctgccgtata 15841 tccttactgg cttccgcaga ggtcaatccg aatatttcag catatttagc aacatggatc 15901 tcgcagatac cgtcatgttc ctgtagggtg ccatcagatt ttctgatctg gtcaacgaac 15961 agatacagca tacgtttttg atcccgggag agactatatg ccgcctcagt gaggtcgttt 16021 gactggacga ttcgcgggct atttttacgt ttcttgtgat tgataaccgc tgtttccgcc 16081 atgacagatc catgtgaagt gtgacaagtt tttagattgt cacactaaat aaaaaagagt 16141 caataagcag ggataacttt gtgaaaaaac agcttcttct gagggcaatt tgtcacaggg 16201 ttaagggcaa tttgtcacag acaggactgt catttgaggg tgatttgtca cactgaaagg 16261 gcaatttgtc acaacacctt ctctagaacc agcatggata aaggcctaca aggcgctcta 16321 aaaaagaaga tctaaaaact ataaaaaaaa taattataaa aatatccccg tggataagtg 16381 gataacccca agggaagttt tttcaggcat cgtgtgtaag cagaatatat aagtgctgtt 16441 ccctggtgct tcctcgctca ctcgagggct tcgccctgtc gctcaactgc ggcgagcact 16501 actggctgta aaaggacaga ccacatcatg gttctgtgtt cattaggttg ttctgtccat 16561 tgctgacata atccgctcca cttcaacgta acaccgcacg aagatttcta ttgttcctga 16621 aggcatattc aaatcgtttt cgttaccgct tgcaggcatc atgacagaac actacttcct 16681 ataaacgcta cacaggctcc tgagattaat aatgcggatc tctacgataa tgggagattt 16741 tcccgactgt ttcgttcgct tctcagtgga taacagccag cttctctgtt taacagacaa 16801 aaacagcata tccactcagt tccacatttc catataaagg ccaaggcatt tattctcagg 16861 ataattgttt cagcatcgca accgcatcag actccggcat cgcaaactgc acccggtgcc 16921 gggcagccac atccagcgca aaaaccttcg tgtagacttc cgttgaactg atggacttat 16981 gtcccatcag gctttgcaga actttcagcg gtataccggc atacagcatg tgcatcgcat 17041 aggaatggcg gaacgtatgt ggtgtgaccg gaacagagaa cgtcacaccg tcagcagcag 17101 cggcggcaac cgcctcccca atccaggtcc tgaccgttct gtccgtcact tcccagatcc 17161 gcgctttctc tgtccttcct gtgcgacggt tacgccgctc catgagctta tcgcgaataa 17221 atacctgtga cggaagatca cttcgcagaa taaataaatc ctggtgtccc tgttgatacc 17281 gggaagccct gggccaactt ttggcgaaaa tgagacgttg atcggcacgt aagaggttcc 17341 aactttcacc ataatgaaat aagatcacta ccgggcgtat tttttgagtt atcgagattt 17401 tcaggagcta aggaagctaa aatggagaaa aaaatcactg gatataccac cgttgatata 17461 tcccaatggc atcgtaaaga acattttgag gcatttcagt cagttgctca atgtacctat 17521 aaccagaccg ttcagctgga tattacggcc tttttaaaga ccgtaaagaa aaataagcac 17581 aagttttatc cggcctttat tcacattctt gcccgcctga tgaatgctca tccggagttc 17641 cgtatggcaa tgaaagacgg tgagctggtg atatgggata gtgttcaccc ttgttacacc 17701 gttttccatg agcaaactga aacgttttca tcgctctgga gtgaatacca cgacgatttc 17761 cggcagtttc tacacatata ttcgcaagat gtggcgtgtt acggtgaaaa cctggcctat 17821 ttccctaaag ggtttattga gaatatgttt ttcgtctcag ccaatccctg ggtgagtttc 17881 accagttttg atttaaacgt ggccaatatg gacaacttct tcgcccccgt tttcaccatg 17941 ggcaaatatt atacgcaagg cgacaaggtg ctgatgccgc tggcgattca ggttcatcat 18001 gccgtttgtg atggcttcca tgtcggcaga atgcttaatg aattacaaca gtactgcgat 18061 gagtggcagg gcggggcgta atttttttaa ggcagttatt ggtgccctta aacgcctggt 18121 tgctacgcct gaataagtga taataagcgg atgaatggca gaaattcgat gataagctgt 18181 caaacatgag aattggtcga cggcgcgcca aagcttgcat gcctgcagcc gcgtaacctg 18241 gcaaaatcgg ttacggttga gtaataaatg gatgccctgc gtaagcgggg cacatttcat 18301 tacctctttc tccgcacccg acatagataa caagtttgta caaaaaagca ggctctagta 18361 gacttaatta aggatcgatc cggcgcgcca atagtcatgc cccgcgccca ccggaaggag 18421 ctgactgggt tgaaggctct caagggcatc ggtcgagctt gacattgtag gactatattg 18481 ctctaataaa tttgcggccg ctaatacgac tcactatagg gagag //