LOCUS Exported 10139 bp ds-DNA circular SYN 01-MAR-2014 DEFINITION pCC1FOS-CHA: a derivative of pCC1FOS with a 2 kb fragment contaning yeast elements inserted into the unique SfiI site. The insert includes the yeast CEN6, His3 and ARSH4 (""CHA"") sequences compatible with yeast propagation & selection as artifical circular YAC. ACCESSION . VERSION . KEYWORDS pCC1FOS-CHA SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 10139) AUTHORS . TITLE Direct Submission JOURNAL Exported Saturday, Mar 01, 2014 from SnapGene 2.2.2 http://www.snapgene.com COMMENT CEN6 has an excelent match (116/117 bp) with CEN6 from Saccharomyces cerevisiae strain W303m (Accession HQ339549.1) FEATURES Location/Qualifiers source 1..10139 /organism="synthetic DNA construct" /mol_type="other DNA" CDS complement(160..447) /codon_start=1 /note="lacZ-alpha" /translation="MTMITPSYLGETIEYSSLHACRSTLEDPTWDPRVPSSNSPYSESY YNSLAVVLQRRDWENPGVTQLNRLAAHPPFASWRNSEEARTDRPSQQLRS" primer_bind 288..304 /note="M13 fwd" /note="common sequencing primer, one of multiple similar variants" promoter 311..329 /note="T7 promoter" /note="promoter for bacteriophage T7 RNA polymerase" primer_bind complement(443..459) /note="M13 rev" /note="common sequencing primer, one of multiple similar variants" protein_bind 467..483 /bound_moiety="lac repressor encoded by lacI" /note="lac operator" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." promoter complement(491..521) /note="lac promoter" /note="promoter for the E. coli lac operon" protein_bind 536..557 /bound_moiety="E. coli catabolite activator protein" /note="CAP binding site" /note="CAP binding activates transcription in the presence of cAMP." misc_feature 651..2650 /note="CEN6-His3-ARSH4-Segment" misc_feature 768..1141 /note="ARSH4" CDS complement(1263..1922) /codon_start=1 /gene="S. cerevisiae HIS3" /product="imidazoleglycerol-phosphate dehydratase, required for histidine biosynthesis" /note="HIS3" /note="yeast auxotrophic marker" /translation="MTEQKALVKRITNETKIQIAISLKGGPLAIEHSIFPEKEAEAVAE QATQSQVINVHTGIGFLDHMIHALAKHSGWSLIVECIGDLHIDDHHTTEDCGIALGQAF KEALLARGVKRFGSGFAPLDEALSRAVVDLSNRPYAVVELGLQREKVGDLSCEMIPHFL ESFAEASRITLHVDCLRGKNDHHRSESAFKALAVAIREATSPNGTNDVPSTKGVLM" promoter complement(1923..2110) /gene="S. cerevisiae HIS3" /note="HIS3 promoter" misc_feature 2500..2616 /note="CEN6" CDS complement(2777..3436) /codon_start=1 /gene="cat" /product="chloramphenicol acetyltransferase" /note="CmR" /note="confers resistance to chloramphenicol" /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA" promoter complement(3437..3539) /note="cat promoter" /note="promoter of the E. coli cat gene" CDS 3655..4002 /codon_start=1 /note="redF" /translation="MERRNRRTGRTEKARIWEVTDRTVRTWIGEAVAAAAADGVTFSVP VTPHTFRHSYAMHMLYAGIPLKVLQSLMGHKSISSTEVYTKVFALDVAARHRVQFAMPE SDAVAMLKQLS" rep_origin 4397..5011 /note="oriV" /note="origin of replication for the bacterial F plasmid" rep_origin 5087..5306 /note="ori2" /note="secondary origin of replication for the bacterial F plasmid; also known as oriS" CDS 5397..6152 /codon_start=1 /gene="repE" /product="replication initiation protein for the bacterial F plasmid" /note="repE" /note=" " /protein_id=" " /translation="MAETAVINHKKRKNSPRIVQSNDLTEAAYSLSRDQKRMLYLFVDQ IRKSDGTLQEHDGICEIHVAKYAEIFGLTSAEASKDIRQALKSFAGKEVVFYRPEEDAG DEKGYESFPWFIKRAHSPSRGLYSVHINPYLIPFFIGLQNRFTQFRLSETKEITNPYAM RLYESLCQYRKPDGSGIVSLKIDWIIERYQLPQSYQRMPDFRRRFLQVCVNEINSRTPM RLSYIEKKKGRQTTHIVFSFRDITSMTTG" misc_feature 6155..6405 /gene="incC" /note="incC" /note="incompatibility region of the bacterial F plasmid" CDS 6731..7906 /codon_start=1 /gene="sopA" /product="partitioning protein for the bacterial F plasmid" /note="sopA" /note=" " /protein_id=" " /translation="MFRMKLMETLNQCINAGHEMTKAIAIAQFNDDSPEARKITRRWRI GEAADLVGVSSQAIRDAEKAGRLPHPDMEIRGRVEQRVGYTIEQINHMRDVFGTRLRRA EDVFPPVIGVAAHKGGVYKTSVSVHLAQDLALKGLRVLLVEGNDPQGTASMYHGWVPDL HIHAEDTLLPFYLGEKDDVTYAIKPTCWPGLDIIPSCLALHRIETELMGKFDEGKLPTD PHLMLRLAIETVAHDYDVIVIDSAPNLGIGTINVVCAADVLIVPTPAELFDYTSALQFF DMLRDLLKNVDLKGFEPDVRILLTKYSNSNGSQSPWMEEQIRDAWGSMVLKNVVRETDE VGKGQIRMRTVFEQAIDQRSSTGAWRNALSIWEPVCNEIFDRLIKPRWEIR" CDS 7906..8877 /codon_start=1 /gene="sopB" /product="partitioning protein for the bacterial F plasmid" /note="sopB" /note=" " /protein_id=" " /translation="MKRAPVIPKHTLNTQPVEDTSLSTPAAPMVDSLIARVGVMARGNA ITLPVCGRDVKFTLEVLRGDSVEKTSRVWSGNERDQELLTEDALDDLIPSFLLTGQQTP AFGRRVSGVIEIADGSRRRKAAALTESDYRVLVGELDDEQMAALSRLGNDYRPTSAYER GQRYASRLQNEFAGNISALADAENISRKIITRCINTAKLPKSVVALFSHPGELSARSGD ALQKAFTDKEELLKQQASNLHEQKKAGVIFEAEEVITLLTSVLKTSSASRTSLSSRHQF APGATVLYKGDKMVLNLDRSRVPTECIEKIEAILKELEKPAP" misc_feature 8950..9423 /gene="sopC" /note="sopC" /note="centromere-like partitioning region of the bacterial F plasmid" misc_feature 9683..10081 /note="cos" /note="lambda cos site; allows packaging into phage lambda particles" protein_bind 10099..10132 /bound_moiety="Cre recombinase" /note="loxP" /note="Cre-mediated recombination occurs in the 8-bp core sequence (GCATACAT)." ORIGIN 1 gcggccgcaa ggggttcgcg tcagcgggtg ttggcgggtg tcggggctgg cttaactatg 61 cggcatcaga gcagattgta ctgagagtgc accatatgcg gtgtgaaata ccgcacagat 121 gcgtaaggag aaaataccgc atcaggcgcc attcgccatt cagctgcgca actgttggga 181 agggcgatcg gtgcgggcct cttcgctatt acgccagctg gcgaaagggg gatgtgctgc 241 aaggcgatta agttgggtaa cgccagggtt ttcccagtca cgacgttgta aaacgacggc 301 cagtgaattg taatacgact cactataggg cgaattcgag ctcggtaccc ggggatccca 361 cgtgggatcc tctagagtcg acctgcaggc atgcaagctt gagtattcta tagtctcacc 421 taaatagctt ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt tatccgctca 481 caattccaca caacatacga gccggaagca taaagtgtaa agcctggggt gcctaatgag 541 tgagctaact cacattaatt gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt 601 cgtgccagct gcattaatga atcggccaac gcgaacccct tgcggccgcc atctgtgcgg 661 tatttcacac cgcatatgat ccgtcgagtt caagagaaaa aaaaagaaaa agcaaaaaga 721 aaaaaggaaa gcgcgcctcg ttcagaatga cacgtataga atgatgcatc gcttgcctgt 781 aacttacacg cgcctcgtat cttttaatga tggaataatt tgggaattta ctctgtgttt 841 atttattttt atgttttgta tttggatttt agaaagtaaa taaagaaggt agaagagtta 901 cggaatgaag aaaaaaaaat aaacaaaggt ttaaaaaatt tcaacaaaaa gcgtacttta 961 catatatatt tattagacaa gaaaagcaga ttaaatagat atacattcga ttaacgataa 1021 gtaaaatgta aaatcacagg attttcgtgt gtggtcttct acacagacaa gatgaaacaa 1081 ttcggcatta atacctgaga gcaggaagag caagataaaa ggtagtattt gttggcgatc 1141 catgcattac cttgtcatct tcagtatcat actgttcgta tacatactta ctgacattca 1201 taggtataca tatatacaca tgtatatata tcgtatgctg cagctttaaa taatcggtgt 1261 cactacataa gaacaccttt ggtggaggga acatcgttgg taccattggg cgaggtggct 1321 tctcttatgg caaccgcaag agccttgaac gcactctcac tacggtgatg atcattcttg 1381 cctcgcagac aatcaacgtg gagggtaatt ctgctagcct ctgcaaagct ttcaagaaaa 1441 tgcgggatca tctcgcaaga gagatctcct actttctccc tttgcaaacc aagttcgaca 1501 actgcgtacg gcctgttcga aagatctacc accgctctgg aaagtgcctc atccaaaggc 1561 gcaaatcctg atccaaacct ttttactcca cgcgccagta gggcctcttt aaaagcttga 1621 ccgagagcaa tcccgcagtc ttcagtggtg tgatggtcgt ctatgtgtaa gtcaccaatg 1681 cactcaacga ttagcgacca gccggaatgc ttggccagag catgtatcat atggtccaga 1741 aaccctatac ctgtgtggac gttaatcact tgcgattgtg tggcctgttc tgctactgct 1801 tctgcctctt tttctgggaa gatcgagtgc tctatcgcta ggggaccacc ctttaaagag 1861 atcgcaatct gaatcttggt ttcatttgta atacgcttta ctagggcttt ctgctctgtc 1921 atctttgcct tcgtttatct tgcctgctca ttttttagta tattcttcga agaaatcaca 1981 ttactttata taatgtataa ttcattatgt gataatgcca atcgctaaga aaaaaaaaga 2041 gtcatccgct aggtggaaaa aaaaaaatga aaatcattac cgaggcataa aaaaatatag 2101 agtgtactag aggaggccaa gagtaataga aaaagaaaat tgcgggaaag gactgtgtta 2161 tgacttccct gactaatgcc gtgttcaaac gatacctggc agtgactcct agcgctcacc 2221 aagctcttaa aacggaatta tggtgcactc tcagtacaat ctgctctgat gccgcatagt 2281 taagccagcc ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc 2341 cggcatccgc ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt 2401 caccgtcatc accgaaacgc gcgagacgaa agggcctcgt gatacgccta tttttatagg 2461 ttaatgtcat gataataatg gtttcttagg gtccttttca tcacatgcta taaaaataat 2521 tataatttaa attttttaat ataaatatat aaattaaaaa tagaaagtaa aaaaagaaat 2581 taaagaaaaa atagtttttg ttttccgaag atgtaaaaga ctctaggggg atcgccaaca 2641 aatactacct cgggccgtcg accaattctc atgtttgaca gcttatcatc gaatttctgc 2701 cattcatccg cttattatca cttattcagg cgtagcaacc aggcgtttaa gggcaccaat 2761 aactgcctta aaaaaattac gccccgccct gccactcatc gcagtactgt tgtaattcat 2821 taagcattct gccgacatgg aagccatcac aaacggcatg atgaacctga atcgccagcg 2881 gcatcagcac cttgtcgcct tgcgtataat atttgcccat ggtgaaaacg ggggcgaaga 2941 agttgtccat attggccacg tttaaatcaa aactggtgaa actcacccag ggattggctg 3001 agacgaaaaa catattctca ataaaccctt tagggaaata ggccaggttt tcaccgtaac 3061 acgccacatc ttgcgaatat atgtgtagaa actgccggaa atcgtcgtgg tattcactcc 3121 agagcgatga aaacgtttca gtttgctcat ggaaaacggt gtaacaaggg tgaacactat 3181 cccatatcac cagctcaccg tctttcattg ccatacgaaa ttccggatga gcattcatca 3241 ggcgggcaag aatgtgaata aaggccggat aaaacttgtg cttatttttc tttacggtct 3301 ttaaaaaggc cgtaatatcc agctgaacgg tctggttata ggtacattga gcaactgact 3361 gaaatgcctc aaaatgttct ttacgatgcc attgggatat atcaacggtg gtatatccag 3421 tgattttttt ctccatttta gcttccttag ctcctgaaaa tctcgataac tcaaaaaata 3481 cgcccggtag tgatcttatt tcattatggt gaaagttgga acctcttacg tgccgatcaa 3541 cgtctcattt tcgccaaaag ttggcccagg gcttcccggt atcaacaggg acaccaggat 3601 ttatttattc tgcgaagtga tcttccgtca caggtattta ttcgcgataa gctcatggag 3661 cggcgtaacc gtcgcacagg aaggacagag aaagcgcgga tctgggaagt gacggacaga 3721 acggtcagga cctggattgg ggaggcggtt gccgccgctg ctgctgacgg tgtgacgttc 3781 tctgttccgg tcacaccaca tacgttccgc cattcctatg cgatgcacat gctgtatgcc 3841 ggtataccgc tgaaagttct gcaaagcctg atgggacata agtccatcag ttcaacggaa 3901 gtctacacga aggtttttgc gctggatgtg gctgcccggc accgggtgca gtttgcgatg 3961 ccggagtctg atgcggttgc gatgctgaaa caattatcct gagaataaat gccttggcct 4021 ttatatggaa atgtggaact gagtggatat gctgtttttg tctgttaaac agagaagctg 4081 gctgttatcc actgagaagc gaacgaaaca gtcgggaaaa tctcccatta tcgtagagat 4141 ccgcattatt aatctcagga gcctgtgtag cgtttatagg aagtagtgtt ctgtcatgat 4201 gcctgcaagc ggtaacgaaa acgatttgaa tatgccttca ggaacaatag aaatcttcgt 4261 gcggtgttac gttgaagtgg agcggattat gtcagcaatg gacagaacaa cctaatgaac 4321 acagaaccat gatgtggtct gtccttttac agccagtagt gctcgccgca gtcgagcgac 4381 agggcgaagc cctcggctgg ttgccctcgc cgctgggctg gcggccgtct atggccctgc 4441 aaacgcgcca gaaacgccgt cgaagccgtg tgcgagacac cgcggccggc cgccggcgtt 4501 gtggatacct cgcggaaaac ttggccctca ctgacagatg aggggcggac gttgacactt 4561 gaggggccga ctcacccggc gcggcgttga cagatgaggg gcaggctcga tttcggccgg 4621 cgacgtggag ctggccagcc tcgcaaatcg gcgaaaacgc ctgattttac gcgagtttcc 4681 cacagatgat gtggacaagc ctggggataa gtgccctgcg gtattgacac ttgaggggcg 4741 cgactactga cagatgaggg gcgcgatcct tgacacttga ggggcagagt gctgacagat 4801 gaggggcgca cctattgaca tttgaggggc tgtccacagg cagaaaatcc agcatttgca 4861 agggtttccg cccgtttttc ggccaccgct aacctgtctt ttaacctgct tttaaaccaa 4921 tatttataaa ccttgttttt aaccagggct gcgccctgtg cgcgtgaccg cgcacgccga 4981 aggggggtgc ccccccttct cgaaccctcc cggtcgagtg agcgaggaag caccagggaa 5041 cagcacttat atattctgct tacacacgat gcctgaaaaa acttcccttg gggttatcca 5101 cttatccacg gggatatttt tataattatt ttttttatag tttttagatc ttctttttta 5161 gagcgccttg taggccttta tccatgctgg ttctagagaa ggtgttgtga caaattgccc 5221 tttcagtgtg acaaatcacc ctcaaatgac agtcctgtct gtgacaaatt gcccttaacc 5281 ctgtgacaaa ttgccctcag aagaagctgt tttttcacaa agttatccct gcttattgac 5341 tcttttttat ttagtgtgac aatctaaaaa cttgtcacac ttcacatgga tctgtcatgg 5401 cggaaacagc ggttatcaat cacaagaaac gtaaaaatag cccgcgaatc gtccagtcaa 5461 acgacctcac tgaggcggca tatagtctct cccgggatca aaaacgtatg ctgtatctgt 5521 tcgttgacca gatcagaaaa tctgatggca ccctacagga acatgacggt atctgcgaga 5581 tccatgttgc taaatatgct gaaatattcg gattgacctc tgcggaagcc agtaaggata 5641 tacggcaggc attgaagagt ttcgcgggga aggaagtggt tttttatcgc cctgaagagg 5701 atgccggcga tgaaaaaggc tatgaatctt ttccttggtt tatcaaacgt gcgcacagtc 5761 catccagagg gctttacagt gtacatatca acccatatct cattcccttc tttatcgggt 5821 tacagaaccg gtttacgcag tttcggctta gtgaaacaaa agaaatcacc aatccgtatg 5881 ccatgcgttt atacgaatcc ctgtgtcagt atcgtaagcc ggatggctca ggcatcgtct 5941 ctctgaaaat cgactggatc atagagcgtt accagctgcc tcaaagttac cagcgtatgc 6001 ctgacttccg ccgccgcttc ctgcaggtct gtgttaatga gatcaacagc agaactccaa 6061 tgcgcctctc atacattgag aaaaagaaag gccgccagac gactcatatc gtattttcct 6121 tccgcgatat cacttccatg acgacaggat agtctgaggg ttatctgtca cagatttgag 6181 ggtggttcgt cacatttgtt ctgacctact gagggtaatt tgtcacagtt ttgctgtttc 6241 cttcagcctg catggatttt ctcatacttt ttgaactgta atttttaagg aagccaaatt 6301 tgagggcagt ttgtcacagt tgatttcctt ctctttccct tcgtcatgtg acctgatatc 6361 gggggttagt tcgtcatcat tgatgagggt tgattatcac agtttattac tctgaattgg 6421 ctatccgcgt gtgtacctct acctggagtt tttcccacgg tggatatttc ttcttgcgct 6481 gagcgtaaga gctatctgac agaacagttc ttctttgctt cctcgccagt tcgctcgcta 6541 tgctcggtta cacggctgcg gcgagcgcta gtgataataa gtgactgagg tatgtgctct 6601 tcttatctcc ttttgtagtg ttgctcttat tttaaacaac tttgcggttt tttgatgact 6661 ttgcgatttt gttgttgctt tgcagtaaat tgcaagattt aataaaaaaa cgcaaagcaa 6721 tgattaaagg atgttcagaa tgaaactcat ggaaacactt aaccagtgca taaacgctgg 6781 tcatgaaatg acgaaggcta tcgccattgc acagtttaat gatgacagcc cggaagcgag 6841 gaaaataacc cggcgctgga gaataggtga agcagcggat ttagttgggg tttcttctca 6901 ggctatcaga gatgccgaga aagcagggcg actaccgcac ccggatatgg aaattcgagg 6961 acgggttgag caacgtgttg gttatacaat tgaacaaatt aatcatatgc gtgatgtgtt 7021 tggtacgcga ttgcgacgtg ctgaagacgt atttccaccg gtgatcgggg ttgctgccca 7081 taaaggtggc gtttacaaaa cctcagtttc tgttcatctt gctcaggatc tggctctgaa 7141 ggggctacgt gttttgctcg tggaaggtaa cgacccccag ggaacagcct caatgtatca 7201 cggatgggta ccagatcttc atattcatgc agaagacact ctcctgcctt tctatcttgg 7261 ggaaaaggac gatgtcactt atgcaataaa gcccacttgc tggccggggc ttgacattat 7321 tccttcctgt ctggctctgc accgtattga aactgagtta atgggcaaat ttgatgaagg 7381 taaactgccc accgatccac acctgatgct ccgactggcc attgaaactg ttgctcatga 7441 ctatgatgtc atagttattg acagcgcgcc taacctgggt atcggcacga ttaatgtcgt 7501 atgtgctgct gatgtgctga ttgttcccac gcctgctgag ttgtttgact acacctccgc 7561 actgcagttt ttcgatatgc ttcgtgatct gctcaagaac gttgatctta aagggttcga 7621 gcctgatgta cgtattttgc ttaccaaata cagcaatagt aatggctctc agtccccgtg 7681 gatggaggag caaattcggg atgcctgggg aagcatggtt ctaaaaaatg ttgtacgtga 7741 aacggatgaa gttggtaaag gtcagatccg gatgagaact gtttttgaac aggccattga 7801 tcaacgctct tcaactggtg cctggagaaa tgctctttct atttgggaac ctgtctgcaa 7861 tgaaattttc gatcgtctga ttaaaccacg ctgggagatt agataatgaa gcgtgcgcct 7921 gttattccaa aacatacgct caatactcaa ccggttgaag atacttcgtt atcgacacca 7981 gctgccccga tggtggattc gttaattgcg cgcgtaggag taatggctcg cggtaatgcc 8041 attactttgc ctgtatgtgg tcgggatgtg aagtttactc ttgaagtgct ccggggtgat 8101 agtgttgaga agacctctcg ggtatggtca ggtaatgaac gtgaccagga gctgcttact 8161 gaggacgcac tggatgatct catcccttct tttctactga ctggtcaaca gacaccggcg 8221 ttcggtcgaa gagtatctgg tgtcatagaa attgccgatg ggagtcgccg tcgtaaagct 8281 gctgcactta ccgaaagtga ttatcgtgtt ctggttggcg agctggatga tgagcagatg 8341 gctgcattat ccagattggg taacgattat cgcccaacaa gtgcttatga acgtggtcag 8401 cgttatgcaa gccgattgca gaatgaattt gctggaaata tttctgcgct ggctgatgcg 8461 gaaaatattt cacgtaagat tattacccgc tgtatcaaca ccgccaaatt gcctaaatca 8521 gttgttgctc ttttttctca ccccggtgaa ctatctgccc ggtcaggtga tgcacttcaa 8581 aaagccttta cagataaaga ggaattactt aagcagcagg catctaacct tcatgagcag 8641 aaaaaagctg gggtgatatt tgaagctgaa gaagttatca ctcttttaac ttctgtgctt 8701 aaaacgtcat ctgcatcaag aactagttta agctcacgac atcagtttgc tcctggagcg 8761 acagtattgt ataagggcga taaaatggtg cttaacctgg acaggtctcg tgttccaact 8821 gagtgtatag agaaaattga ggccattctt aaggaacttg aaaagccagc accctgatgc 8881 gaccacgttt tagtctacgt ttatctgtct ttacttaatg tcctttgtta caggccagaa 8941 agcataactg gcctgaatat tctctctggg cccactgttc cacttgtatc gtcggtctga 9001 taatcagact gggaccacgg tcccactcgt atcgtcggtc tgattattag tctgggacca 9061 cggtcccact cgtatcgtcg gtctgattat tagtctggga ccacggtccc actcgtatcg 9121 tcggtctgat aatcagactg ggaccacggt cccactcgta tcgtcggtct gattattagt 9181 ctgggaccat ggtcccactc gtatcgtcgg tctgattatt agtctgggac cacggtccca 9241 ctcgtatcgt cggtctgatt attagtctgg aaccacggtc ccactcgtat cgtcggtctg 9301 attattagtc tgggaccacg gtcccactcg tatcgtcggt ctgattatta gtctgggacc 9361 acgatcccac tcgtgttgtc ggtctgatta tcggtctggg accacggtcc cacttgtatt 9421 gtcgatcaga ctatcagcgt gagactacga ttccatcaat gcctgtcaag ggcaagtatt 9481 gacatgtcgt cgtaacctgt agaacggagt aacctcggtg tgcggttgta tgcctgctgt 9541 ggattgctgc tgtgtcctgc ttatccacaa cattttgcgc acggttatgt ggacaaaata 9601 cctggttacc caggccgtgc cggcacgtta accgggctgc atccgatgca agtgtgtcgc 9661 tgtcgacgag ctcgcgagct cggacatgag gttgccccgt attcagtgtc gctgatttgt 9721 attgtctgaa gttgttttta cgttaagttg atgcagatca attaatacga tacctgcgtc 9781 ataattgatt atttgacgtg gtttgatggc ctccacgcac gttgtgatat gtagatgata 9841 atcattatca ctttacgggt cctttccggt gatccgacag gttacggggc ggcgacctcg 9901 cgggttttcg ctatttatga aaattttccg gtttaaggcg tttccgttct tcttcgtcat 9961 aacttaatgt ttttatttaa aataccctct gaaaagaaag gaaacgacag gtgctgaaag 10021 cgagcttttt ggcctctgtc gtttcctttc tctgtttttg tccgtggaat gaacaatgga 10081 agtccgagct catcgctaat aacttcgtat agcatacatt atacgaagtt atattcgat //