LOCUS Exported 8181 bp ds-DNA circular SYN 01-MAR-2014 DEFINITION . ACCESSION . VERSION . KEYWORDS pCC1FOS SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 8181) AUTHORS . TITLE Direct Submission JOURNAL Exported Saturday, Mar 01, 2014 from SnapGene 2.2.2 http://www.snapgene.com FEATURES Location/Qualifiers source 1..8181 /organism="synthetic DNA construct" /lab_host="Escherichia coli" /mol_type="other DNA" CDS complement(160..489) /codon_start=1 /note="lacZ-alpha" /translation="MTMITPSYLGETIEYSSLHACRSTLEDPTRKQPRNTWSRCRCTWD PRVPSSNSPYSESYYNSLAVVLQRRDWENPGVTQLNRLAAHPPFASWRNSEEARTDRPS QQLRS" primer_bind 288..304 /note="M13 fwd" /note="common sequencing primer, one of multiple similar variants" promoter 311..329 /note="T7 promoter" /note="promoter for bacteriophage T7 RNA polymerase" primer_bind complement(485..501) /note="M13 rev" /note="common sequencing primer, one of multiple similar variants" protein_bind 509..525 /bound_moiety="lac repressor encoded by lacI" /note="lac operator" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." promoter complement(533..563) /note="lac promoter" /note="promoter for the E. coli lac operon" protein_bind 578..599 /bound_moiety="E. coli catabolite activator protein" /note="CAP binding site" /note="CAP binding activates transcription in the presence of cAMP." CDS complement(819..1478) /codon_start=1 /gene="cat" /product="chloramphenicol acetyltransferase" /note="CmR" /note="confers resistance to chloramphenicol" /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA" promoter complement(1479..1581) /note="cat promoter" /note="promoter of the E. coli cat gene" CDS 1697..2044 /codon_start=1 /note="redF" /translation="MERRNRRTGRTEKARIWEVTDRTVRTWIGEAVAAAAADGVTFSVP VTPHTFRHSYAMHMLYAGIPLKVLQSLMGHKSISSTEVYTKVFALDVAARHRVQFAMPE SDAVAMLKQLS" rep_origin 2439..3053 /note="oriV" /note="origin of replication for the bacterial F plasmid" rep_origin 3129..3348 /note="ori2" /note="secondary origin of replication for the bacterial F plasmid; also known as oriS" CDS 3439..4194 /codon_start=1 /gene="repE" /product="replication initiation protein for the bacterial F plasmid" /note="repE" /note=" " /protein_id=" " /translation="MAETAVINHKKRKNSPRIVQSNDLTEAAYSLSRDQKRMLYLFVDQ IRKSDGTLQEHDGICEIHVAKYAEIFGLTSAEASKDIRQALKSFAGKEVVFYRPEEDAG DEKGYESFPWFIKRAHSPSRGLYSVHINPYLIPFFIGLQNRFTQFRLSETKEITNPYAM RLYESLCQYRKPDGSGIVSLKIDWIIERYQLPQSYQRMPDFRRRFLQVCVNEINSRTPM RLSYIEKKKGRQTTHIVFSFRDITSMTTG" misc_feature 4197..4447 /gene="incC" /note="incC" /note="incompatibility region of the bacterial F plasmid" CDS 4773..5948 /codon_start=1 /gene="sopA" /product="partitioning protein for the bacterial F plasmid" /note="sopA" /note=" " /protein_id=" " /translation="MFRMKLMETLNQCINAGHEMTKAIAIAQFNDDSPEARKITRRWRI GEAADLVGVSSQAIRDAEKAGRLPHPDMEIRGRVEQRVGYTIEQINHMRDVFGTRLRRA EDVFPPVIGVAAHKGGVYKTSVSVHLAQDLALKGLRVLLVEGNDPQGTASMYHGWVPDL HIHAEDTLLPFYLGEKDDVTYAIKPTCWPGLDIIPSCLALHRIETELMGKFDEGKLPTD PHLMLRLAIETVAHDYDVIVIDSAPNLGIGTINVVCAADVLIVPTPAELFDYTSALQFF DMLRDLLKNVDLKGFEPDVRILLTKYSNSNGSQSPWMEEQIRDAWGSMVLKNVVRETDE VGKGQIRMRTVFEQAIDQRSSTGAWRNALSIWEPVCNEIFDRLIKPRWEIR" CDS 5948..6919 /codon_start=1 /gene="sopB" /product="partitioning protein for the bacterial F plasmid" /note="sopB" /note=" " /protein_id=" " /translation="MKRAPVIPKHTLNTQPVEDTSLSTPAAPMVDSLIARVGVMARGNA ITLPVCGRDVKFTLEVLRGDSVEKTSRVWSGNERDQELLTEDALDDLIPSFLLTGQQTP AFGRRVSGVIEIADGSRRRKAAALTESDYRVLVGELDDEQMAALSRLGNDYRPTSAYER GQRYASRLQNEFAGNISALADAENISRKIITRCINTAKLPKSVVALFSHPGELSARSGD ALQKAFTDKEELLKQQASNLHEQKKAGVIFEAEEVITLLTSVLKTSSASRTSLSSRHQF APGATVLYKGDKMVLNLDRSRVPTECIEKIEAILKELEKPAP" misc_feature 6992..7465 /gene="sopC" /note="sopC" /note="centromere-like partitioning region of the bacterial F plasmid" misc_feature 7725..8123 /note="cos" /note="lambda cos site; allows packaging into phage lambda particles" protein_bind 8141..8174 /bound_moiety="Cre recombinase" /note="loxP" /note="Cre-mediated recombination occurs in the 8-bp core sequence (GCATACAT)." ORIGIN 1 gcggccgcaa ggggttcgcg tcagcgggtg ttggcgggtg tcggggctgg cttaactatg 61 cggcatcaga gcagattgta ctgagagtgc accatatgcg gtgtgaaata ccgcacagat 121 gcgtaaggag aaaataccgc atcaggcgcc attcgccatt cagctgcgca actgttggga 181 agggcgatcg gtgcgggcct cttcgctatt acgccagctg gcgaaagggg gatgtgctgc 241 aaggcgatta agttgggtaa cgccagggtt ttcccagtca cgacgttgta aaacgacggc 301 cagtgaattg taatacgact cactataggg cgaattcgag ctcggtaccc ggggatccca 361 cgtacaacga cacctagacc acgtgttcct aggctgtttc ctggtgggat cctctagagt 421 cgacctgcag gcatgcaagc ttgagtattc tatagtctca cctaaatagc ttggcgtaat 481 catggtcata gctgtttcct gtgtgaaatt gttatccgct cacaattcca cacaacatac 541 gagccggaag cataaagtgt aaagcctggg gtgcctaatg agtgagctaa ctcacattaa 601 ttgcgttgcg ctcactgccc gctttccagt cgggaaacct gtcgtgccag ctgcattaat 661 gaatcggcca acgcgaaccc cttgcggccg cccgggccgt cgaccaattc tcatgtttga 721 cagcttatca tcgaatttct gccattcatc cgcttattat cacttattca ggcgtagcaa 781 ccaggcgttt aagggcacca ataactgcct taaaaaaatt acgccccgcc ctgccactca 841 tcgcagtact gttgtaattc attaagcatt ctgccgacat ggaagccatc acaaacggca 901 tgatgaacct gaatcgccag cggcatcagc accttgtcgc cttgcgtata atatttgccc 961 atggtgaaaa cgggggcgaa gaagttgtcc atattggcca cgtttaaatc aaaactggtg 1021 aaactcaccc agggattggc tgagacgaaa aacatattct caataaaccc tttagggaaa 1081 taggccaggt tttcaccgta acacgccaca tcttgcgaat atatgtgtag aaactgccgg 1141 aaatcgtcgt ggtattcact ccagagcgat gaaaacgttt cagtttgctc atggaaaacg 1201 gtgtaacaag ggtgaacact atcccatatc accagctcac cgtctttcat tgccatacga 1261 aattccggat gagcattcat caggcgggca agaatgtgaa taaaggccgg ataaaacttg 1321 tgcttatttt tctttacggt ctttaaaaag gccgtaatat ccagctgaac ggtctggtta 1381 taggtacatt gagcaactga ctgaaatgcc tcaaaatgtt ctttacgatg ccattgggat 1441 atatcaacgg tggtatatcc agtgattttt ttctccattt tagcttcctt agctcctgaa 1501 aatctcgata actcaaaaaa tacgcccggt agtgatctta tttcattatg gtgaaagttg 1561 gaacctctta cgtgccgatc aacgtctcat tttcgccaaa agttggccca gggcttcccg 1621 gtatcaacag ggacaccagg atttatttat tctgcgaagt gatcttccgt cacaggtatt 1681 tattcgcgat aagctcatgg agcggcgtaa ccgtcgcaca ggaaggacag agaaagcgcg 1741 gatctgggaa gtgacggaca gaacggtcag gacctggatt ggggaggcgg ttgccgccgc 1801 tgctgctgac ggtgtgacgt tctctgttcc ggtcacacca catacgttcc gccattccta 1861 tgcgatgcac atgctgtatg ccggtatacc gctgaaagtt ctgcaaagcc tgatgggaca 1921 taagtccatc agttcaacgg aagtctacac gaaggttttt gcgctggatg tggctgcccg 1981 gcaccgggtg cagtttgcga tgccggagtc tgatgcggtt gcgatgctga aacaattatc 2041 ctgagaataa atgccttggc ctttatatgg aaatgtggaa ctgagtggat atgctgtttt 2101 tgtctgttaa acagagaagc tggctgttat ccactgagaa gcgaacgaaa cagtcgggaa 2161 aatctcccat tatcgtagag atccgcatta ttaatctcag gagcctgtgt agcgtttata 2221 ggaagtagtg ttctgtcatg atgcctgcaa gcggtaacga aaacgatttg aatatgcctt 2281 caggaacaat agaaatcttc gtgcggtgtt acgttgaagt ggagcggatt atgtcagcaa 2341 tggacagaac aacctaatga acacagaacc atgatgtggt ctgtcctttt acagccagta 2401 gtgctcgccg cagtcgagcg acagggcgaa gccctcggct ggttgccctc gccgctgggc 2461 tggcggccgt ctatggccct gcaaacgcgc cagaaacgcc gtcgaagccg tgtgcgagac 2521 accgcggccg gccgccggcg ttgtggatac ctcgcggaaa acttggccct cactgacaga 2581 tgaggggcgg acgttgacac ttgaggggcc gactcacccg gcgcggcgtt gacagatgag 2641 gggcaggctc gatttcggcc ggcgacgtgg agctggccag cctcgcaaat cggcgaaaac 2701 gcctgatttt acgcgagttt cccacagatg atgtggacaa gcctggggat aagtgccctg 2761 cggtattgac acttgagggg cgcgactact gacagatgag gggcgcgatc cttgacactt 2821 gaggggcaga gtgctgacag atgaggggcg cacctattga catttgaggg gctgtccaca 2881 ggcagaaaat ccagcatttg caagggtttc cgcccgtttt tcggccaccg ctaacctgtc 2941 ttttaacctg cttttaaacc aatatttata aaccttgttt ttaaccaggg ctgcgccctg 3001 tgcgcgtgac cgcgcacgcc gaaggggggt gccccccctt ctcgaaccct cccggtcgag 3061 tgagcgagga agcaccaggg aacagcactt atatattctg cttacacacg atgcctgaaa 3121 aaacttccct tggggttatc cacttatcca cggggatatt tttataatta ttttttttat 3181 agtttttaga tcttcttttt tagagcgcct tgtaggcctt tatccatgct ggttctagag 3241 aaggtgttgt gacaaattgc cctttcagtg tgacaaatca ccctcaaatg acagtcctgt 3301 ctgtgacaaa ttgcccttaa ccctgtgaca aattgccctc agaagaagct gttttttcac 3361 aaagttatcc ctgcttattg actctttttt atttagtgtg acaatctaaa aacttgtcac 3421 acttcacatg gatctgtcat ggcggaaaca gcggttatca atcacaagaa acgtaaaaat 3481 agcccgcgaa tcgtccagtc aaacgacctc actgaggcgg catatagtct ctcccgggat 3541 caaaaacgta tgctgtatct gttcgttgac cagatcagaa aatctgatgg caccctacag 3601 gaacatgacg gtatctgcga gatccatgtt gctaaatatg ctgaaatatt cggattgacc 3661 tctgcggaag ccagtaagga tatacggcag gcattgaaga gtttcgcggg gaaggaagtg 3721 gttttttatc gccctgaaga ggatgccggc gatgaaaaag gctatgaatc ttttccttgg 3781 tttatcaaac gtgcgcacag tccatccaga gggctttaca gtgtacatat caacccatat 3841 ctcattccct tctttatcgg gttacagaac cggtttacgc agtttcggct tagtgaaaca 3901 aaagaaatca ccaatccgta tgccatgcgt ttatacgaat ccctgtgtca gtatcgtaag 3961 ccggatggct caggcatcgt ctctctgaaa atcgactgga tcatagagcg ttaccagctg 4021 cctcaaagtt accagcgtat gcctgacttc cgccgccgct tcctgcaggt ctgtgttaat 4081 gagatcaaca gcagaactcc aatgcgcctc tcatacattg agaaaaagaa aggccgccag 4141 acgactcata tcgtattttc cttccgcgat atcacttcca tgacgacagg atagtctgag 4201 ggttatctgt cacagatttg agggtggttc gtcacatttg ttctgaccta ctgagggtaa 4261 tttgtcacag ttttgctgtt tccttcagcc tgcatggatt ttctcatact ttttgaactg 4321 taatttttaa ggaagccaaa tttgagggca gtttgtcaca gttgatttcc ttctctttcc 4381 cttcgtcatg tgacctgata tcgggggtta gttcgtcatc attgatgagg gttgattatc 4441 acagtttatt actctgaatt ggctatccgc gtgtgtacct ctacctggag tttttcccac 4501 ggtggatatt tcttcttgcg ctgagcgtaa gagctatctg acagaacagt tcttctttgc 4561 ttcctcgcca gttcgctcgc tatgctcggt tacacggctg cggcgagcgc tagtgataat 4621 aagtgactga ggtatgtgct cttcttatct ccttttgtag tgttgctctt attttaaaca 4681 actttgcggt tttttgatga ctttgcgatt ttgttgttgc tttgcagtaa attgcaagat 4741 ttaataaaaa aacgcaaagc aatgattaaa ggatgttcag aatgaaactc atggaaacac 4801 ttaaccagtg cataaacgct ggtcatgaaa tgacgaaggc tatcgccatt gcacagttta 4861 atgatgacag cccggaagcg aggaaaataa cccggcgctg gagaataggt gaagcagcgg 4921 atttagttgg ggtttcttct caggctatca gagatgccga gaaagcaggg cgactaccgc 4981 acccggatat ggaaattcga ggacgggttg agcaacgtgt tggttataca attgaacaaa 5041 ttaatcatat gcgtgatgtg tttggtacgc gattgcgacg tgctgaagac gtatttccac 5101 cggtgatcgg ggttgctgcc cataaaggtg gcgtttacaa aacctcagtt tctgttcatc 5161 ttgctcagga tctggctctg aaggggctac gtgttttgct cgtggaaggt aacgaccccc 5221 agggaacagc ctcaatgtat cacggatggg taccagatct tcatattcat gcagaagaca 5281 ctctcctgcc tttctatctt ggggaaaagg acgatgtcac ttatgcaata aagcccactt 5341 gctggccggg gcttgacatt attccttcct gtctggctct gcaccgtatt gaaactgagt 5401 taatgggcaa atttgatgaa ggtaaactgc ccaccgatcc acacctgatg ctccgactgg 5461 ccattgaaac tgttgctcat gactatgatg tcatagttat tgacagcgcg cctaacctgg 5521 gtatcggcac gattaatgtc gtatgtgctg ctgatgtgct gattgttccc acgcctgctg 5581 agttgtttga ctacacctcc gcactgcagt ttttcgatat gcttcgtgat ctgctcaaga 5641 acgttgatct taaagggttc gagcctgatg tacgtatttt gcttaccaaa tacagcaata 5701 gtaatggctc tcagtccccg tggatggagg agcaaattcg ggatgcctgg ggaagcatgg 5761 ttctaaaaaa tgttgtacgt gaaacggatg aagttggtaa aggtcagatc cggatgagaa 5821 ctgtttttga acaggccatt gatcaacgct cttcaactgg tgcctggaga aatgctcttt 5881 ctatttggga acctgtctgc aatgaaattt tcgatcgtct gattaaacca cgctgggaga 5941 ttagataatg aagcgtgcgc ctgttattcc aaaacatacg ctcaatactc aaccggttga 6001 agatacttcg ttatcgacac cagctgcccc gatggtggat tcgttaattg cgcgcgtagg 6061 agtaatggct cgcggtaatg ccattacttt gcctgtatgt ggtcgggatg tgaagtttac 6121 tcttgaagtg ctccggggtg atagtgttga gaagacctct cgggtatggt caggtaatga 6181 acgtgaccag gagctgctta ctgaggacgc actggatgat ctcatccctt cttttctact 6241 gactggtcaa cagacaccgg cgttcggtcg aagagtatct ggtgtcatag aaattgccga 6301 tgggagtcgc cgtcgtaaag ctgctgcact taccgaaagt gattatcgtg ttctggttgg 6361 cgagctggat gatgagcaga tggctgcatt atccagattg ggtaacgatt atcgcccaac 6421 aagtgcttat gaacgtggtc agcgttatgc aagccgattg cagaatgaat ttgctggaaa 6481 tatttctgcg ctggctgatg cggaaaatat ttcacgtaag attattaccc gctgtatcaa 6541 caccgccaaa ttgcctaaat cagttgttgc tcttttttct caccccggtg aactatctgc 6601 ccggtcaggt gatgcacttc aaaaagcctt tacagataaa gaggaattac ttaagcagca 6661 ggcatctaac cttcatgagc agaaaaaagc tggggtgata tttgaagctg aagaagttat 6721 cactctttta acttctgtgc ttaaaacgtc atctgcatca agaactagtt taagctcacg 6781 acatcagttt gctcctggag cgacagtatt gtataagggc gataaaatgg tgcttaacct 6841 ggacaggtct cgtgttccaa ctgagtgtat agagaaaatt gaggccattc ttaaggaact 6901 tgaaaagcca gcaccctgat gcgaccacgt tttagtctac gtttatctgt ctttacttaa 6961 tgtcctttgt tacaggccag aaagcataac tggcctgaat attctctctg ggcccactgt 7021 tccacttgta tcgtcggtct gataatcaga ctgggaccac ggtcccactc gtatcgtcgg 7081 tctgattatt agtctgggac cacggtccca ctcgtatcgt cggtctgatt attagtctgg 7141 gaccacggtc ccactcgtat cgtcggtctg ataatcagac tgggaccacg gtcccactcg 7201 tatcgtcggt ctgattatta gtctgggacc atggtcccac tcgtatcgtc ggtctgatta 7261 ttagtctggg accacggtcc cactcgtatc gtcggtctga ttattagtct ggaaccacgg 7321 tcccactcgt atcgtcggtc tgattattag tctgggacca cggtcccact cgtatcgtcg 7381 gtctgattat tagtctggga ccacgatccc actcgtgttg tcggtctgat tatcggtctg 7441 ggaccacggt cccacttgta ttgtcgatca gactatcagc gtgagactac gattccatca 7501 atgcctgtca agggcaagta ttgacatgtc gtcgtaacct gtagaacgga gtaacctcgg 7561 tgtgcggttg tatgcctgct gtggattgct gctgtgtcct gcttatccac aacattttgc 7621 gcacggttat gtggacaaaa tacctggtta cccaggccgt gccggcacgt taaccgggct 7681 gcatccgatg caagtgtgtc gctgtcgacg agctcgcgag ctcggacatg aggttgcccc 7741 gtattcagtg tcgctgattt gtattgtctg aagttgtttt tacgttaagt tgatgcagat 7801 caattaatac gatacctgcg tcataattga ttatttgacg tggtttgatg gcctccacgc 7861 acgttgtgat atgtagatga taatcattat cactttacgg gtcctttccg gtgatccgac 7921 aggttacggg gcggcgacct cgcgggtttt cgctatttat gaaaattttc cggtttaagg 7981 cgtttccgtt cttcttcgtc ataacttaat gtttttattt aaaataccct ctgaaaagaa 8041 aggaaacgac aggtgctgaa agcgagcttt ttggcctctg tcgtttcctt tctctgtttt 8101 tgtccgtgga atgaacaatg gaagtccgag ctcatcgcta ataacttcgt atagcataca 8161 ttatacgaag ttatattcga t //