ƚEJV !$  Q---$$^<(>Vpp[I9," /Ru )4CU=iZu1Sv$EdfJ, &*+a)>#|aH1uV 6 ,Lj2H`z 7#Z#| !?[ugH)];ut[`?N"?2)#}Z"8(1=K]q{aH36Xz--+#--- 02Arialw@\$ ww w0- .2 h2pTARBAC4 (updated)."System &$w- 02Arialw@\$ ww w0- . 2 |13466 bp.- -f--*$ "+25}6Y35.!Bc - . 6  !*!. --- 02Arialw@\$ ww w0- j2. 2 kSACBII.-- -f--.$h^;E4&"F j,*m+K/*6 @M]^- E6  ,^*,^h^;E--- 02Arialw@\$ ww w0- .2  Yeast His3 cassette.-- -f--"$M^AD!7%!AK- D6  7K*7KM^AD--- 02Arialw@\$ ww w0- Z. 2 CM(R)I.-- -%6-- 02Arialw@\$ ww w0- [n6. 2 woSP6R.-- -%-- 02Arialw@\$ ww w0- &t.2 &[T7.-- -%tQ-- 02Arialw@\$ ww w0- t. 2 LOXP.-- -%x~-- 02Arialw@\$ ww w0- eOx. 2 eLOXP511p.-- -%qx-- 02Arialw@\$ ww w0- Mq. 2 PI_SCEIp.-- -%em-- 02Arialw@\$ ww w0- e. 2 TN7ATT.-- ---$S]@n"Q S3!-<Ncz}m_T L+HLGnINWbq,F_v<]~#A^yxaS?uU]iCz( tV9{jWA*yohedafBl$tq_O,CI9Z4- SSG ?4Z,d?S4Z@]"n QS--- 02Arialw@\$ ww w0- t p. 2 PBAC108L.-- ---B$ksUWB:2%,.4<!G<UVeox- G ,,d,--- 02Arialw@\$ ww w0-  Yw. 2 PUC-LINK.-- ---$ :6/.- 6  6.*6.:--- 02Arialw@\$ ww w0- . 2 X Yeast CEN6.-- -% S-- 02Arialw@\$ ww w0-  . 2 ORI-.-- -%--0Times New Romanww w0- . 2  BamHI (1)6.-- -%6--0Times New Romanww w0- @t6. 2 Tu BamHI (2811).-- -%}--0Times New Romanww w0- . 2  NcoI (5321)).-- -%-= --0Times New Romanww w0- z-. 2  NcoI (12864).-- -%2--0Times New Romanww w0- l2. 2 m SmaI (2746)).-- -%/+`--0Times New Romanww w0- .+/. 2 . SmaI (9062)).-- -%2--0Times New Romanww w0- g2. 2 h XmaI (2744)).-- -%/+`--0Times New Romanww w0- Y+/. 2 m XmaI (9060)).-- -%2--0Times New Romanww w0- c2. 2 d AvaI (2744)).-- -%--0Times New Romanww w0- 3. 2 G AvaI (6434)).-- -%/+`--0Times New Romanww w0- :+/. 2 N AvaI (9060)).-- -%--0Times New Romanww w0- . 2  EcoRI (10).-- -%1--0Times New Romanww w0- g1. 2 h EcoRI (2728).-- -%5 --0Times New Romanww w0- !p5. 2 5q EcoRI (2802).-- -%7#--0Times New Romanww w0- 7. 2  ApaLI (766)).-- -%u--0Times New Romanww w0- Z+u. 2 Z, ApaLI (2012).-- -%#--0Times New Romanww w0- Z#. 2 [ ApaLI (2509).-- -%d7d--0Times New Romanww w0- _d.2 s` ApaLI (10123).-- -%?--0Times New Romanww w0- o?. 2 p ClaI (3630)3.-- -%.--0Times New Romanww w0- `.. 2 a ClaI (4032)3.-- -%lI--0Times New Romanww w0- x!l. 2 " ClaI (4784)3.-- -%--0Times New Romanww w0- BW. 2 B ClaI (13312).-- -%--0Times New Romanww w0- R. 2 S PstI (85)1.-- -%@7 >--0Times New Romanww w0- ~q7@. 2 r PstI (3093)).-- -%mt--0Times New Romanww w0- 3Em. 2 G PstI (6993)).-- -%iZ--0Times New Romanww w0- 3i. 2  PstI (8534)).-- -%0a--0Times New Romanww w0- 0. 2  PstI (11137).-- -%em--0Times New Romanww w0- <e. 2  PstI (13155).-- -%--0Times New Romanww w0- . 2  HindIII (93).-- -%9--0Times New Romanww w0- k9.2 lHindIII (3821).-- -%--0Times New Romanww w0- R.2 .SHindIII (4270).-- -%kH--0Times New Romanww w0- m5k.2 m6HindIII (4777).-- -%H--0Times New Romanww w0- .2 fHindIII (10755).-- -% S--0Times New Romanww w0-  .2 uHindIII (10945).-- -%bj--0Times New Romanww w0- b.2 2HindIII (13139).--