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Questions
and Answers (continued)
12)
I would like to order a mouse BAC with the name MSMg01-88E21.
The BACPAC shopping cart does not recognize this clone. Why?
The
MSMG BACs are from a BAC library created by Dr.Abe in Japan.
These BACs are not derived from one of the common laboratory
strains. The library name indicates the name of the sub-species:
MSM/Ms is an inbred strain derived from the Japanese wild
mouse, Mus musculus molossinus. The library and specific BACs
are only available from a Japanese distributor.
See
the publication :
http://www.ncbi.nlm.nih.gov/pubmed/15574823
?
13)
I have seen BAC clones from the NOD mouse strain in ENSEMBL.
Does BACPAC distributes these? The BACPAC shopping cart does
not recognize " bCN538a05".
Mapped BAC clones
from the CHORI-29 BAC library can currently (January 2010)
be found in two ways: 1)Displayed in the ENSEMBL (after configuring
the browser display), or 2)By searching the NCBI CloneFinder
(Cf) program. Please note that these databases follow
different naming "dialects" for the BAC clones. The clones
found through ENSEMBL
have the following format: "bCN538a05". The same clone in
CloneFinder is named: "CH29-538A5", which is the NCBI standard
nomenclature used for more 10 years. BACPAC Resources has
an online ordering system and this uses the NCBI Nomenclature.
Therefore, clone names found on the ENSEMBL site should be
translated into NCBI format to be recognized by the BACPAC
electronic shopping cart. ["bCN" becomes "CH29-" and an
extra digit in the "column" number should be removed: "05"
should become "5"]. Additional information related to the
NOD BAC library can be found here.
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