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Knock Out Mouse Poject (KOMP) at CHORI

KOMP is a trans-NIH initiative to generate a public resource of mouse embryonic stem (ES) cells containing a null mutation in every gene in the mouse genome. Two groups have been funded by this program to generate knockout mouse lines:

• Regeneron Pharmaceuticals, Inc.

• a collaborative team (CSD-KOMP) at the Children's Hospital Oakland Research Institute, the University of California at Davis School of Veterinary Medicine , and the Wellcome Trust Sanger Institute . Groups at the University of Pennsylvania , the Samuel Lunenfeld Research Institute of Mount Sinai Hospital, and at Regeneron Pharmaceuticals, Inc. have been funded to develop methods to improve the efficiency of creating knockout C57BL/6 ES cell lines.
  
  For more details see NIH KOMP Home Page

• Regeneron generates null KO alleles with deletions of the major portion of the gene of interest.
• CSD-KOMP generates flexible conditional KO with or without reporter gene
  
  • Detail of the CSD-KOMP strategy
    

• Quality assessment of the pre-Gateway and post-Gateway constructs
  

• Involved in CSD KOMP strategy
• Creation of the pre-Gateway vectors
• Distribution of pre-Gateway and post-Gateway vectors
  

BACPAC Resources supplies pre-gateway intermediate clones and post-gateway constructs ready for ES cells transformation stored in e.coli.

Please follow the link to our KOMP ordering page to find and order the vector for your gene of interest. Please note, komp orders will be processed through the BACPAC Resources Center (BPRC) online ordering system.

For KOMP ES cell lines and cryopreserved embryos, please use the UC Davis KOMP ordering website.

Search our repository of KOMP genes we have processed so far: