Non-recombinant
clones in the library containing the intact vector were identified
and distinguished from clones containing insert by colony
hybridization using the vector as a probe. Non-recombinant
clones contain the high-copy number pUC replicon, lacking
in the recombinant clones, thus resulting in a much higher
hybridization signal of vector sequences.
It should be noted
that while analyzing BAC clones using pulsed-field gel electrophoresis
to determine the average insert size, non-insert clones containing
a small deleted vector fragment consistent with sucrose resistance
were observed.
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