| ISB-1
Xenopus tropicalis BAC library
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The ISB-1 Xenopus tropicalis BAC library has been constructed
by Shizhen Qin*, Monica Dors, Brian Birditt,
and Jeremy Burke in Leroy Hood Laboratory at Institute for
System Biology. High-molecular-weight DNA was isolated from
blood which was obtained from a female Xenopus tropicalis
through Dr. Robert Grainger (University of Virginia). DNA
was isolated, partially digested with HindIII or MboI and
cloned at the HindIII (Segments 1) or BamHI (Segments 2) site
of the pBeloBAC11 vector. The ligation products were transformed
into DH10B electrocompetent cells (GeneHogs, Invitrogen).
The libraries have been arrayed into 384-well microtiter dishes.
Library construction was funded in part by a grant form the
National Human Genome Research Institute (P50 HG01791).
| Segment |
Cloning Vector |
DNA |
Restriction enzyme |
Plate Number |
Total Plates |
Empty Wells |
Empty Wells (%) |
| 1 |
pBeloBAC11 |
Female Whole Blood |
HindIII |
1-200 |
200 |
1,536 |
2% |
| 2 |
pBeloBAC11 |
Female Whole Blood |
MboI |
201-400 |
200 |
1,536 |
2% |
| Total Library |
|
Female Whole Blood |
|
1-400 |
400 |
3,072 |
2% |
| Segment |
Non-insert clones (%) |
Non-insert clones |
recombinant clones |
Insert Size (Average) |
Redundancy (Genome Size: 1.6Gb) |
| 1 |
1% |
Approx.768 |
Approx. 74,496 |
75 Kbp |
3.4X |
| 2 |
1% |
Approx.768 |
Approx. 74,496 |
75 Kbp |
3.4X |
| Total library |
1% |
Approx.1,576 |
Approx. 148,992 |
75 Kbp |
6.8X |
click here
for a legend of the previous tables.
Data on the ISB-1 Xenopus tropicalis BAC library clone average
insert size has been determined by Pulsed Field Gel Electrophoresis.
Clone size distribution has been
plotted graphically.
Individual clones annotated in databases or screened in any
way, can be obtained as stab cultures at a cost of $80 per clone in addition to a $35 set-up charge per shipment. Shipping
will be done by Federal Express and charged to the recipients
account number. We are not planning to prepare high-density
filters unless we become aware of some sustained interest
for this. Please realize that there may thus be a minor delay
obtaining filters as a consequence of not having filters in
stock. Single clone orders can be obtained according to the
distribution policy of BACPAC Resources.
With respect to the scheduling of shipments of arrayed library
copies, we would like to make you aware of our policy
for array distribution.
Please visit the ordering information
page if you want to place an order.
Academic and commercial users interested in a copy of the BAC library should
contact Pieter J. de Jong ([email protected], fax: (510) 450-7924). For hybridization
membranes, please contact BACPAC Resources ([email protected]).
*Shizhen Qin, Ph.D.
The Institute for Systems Biology
1441 North 34th Street
Seattle, WA 98103
[email protected]
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