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CHORI-259: Common Marmoset BAC Library |
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The CHORI-259 Common Marmoset (Callithrix jacchus, male) BAC library has been constructed in Pieter De Jong's Laboratoryat the Children’s Hospital Oakland Research Institute, BACPAC Resources, by Doriana Misceo and Dr. Baoli Zhu usingthe cloning techniques developed in our laboratory (Osoegawa et al., 1998). The marmoset sample for this library waskindly provided by Dr.Suzette Tardif, Associate Director of the Southwest National Primate Research Center in San Antonio TX. DNA was isolated fromkidney cells obtained from a male marmoset(SNPRC #17081). Another sample from the same animal has been used to establisha permanent cell line which can be obtained from the IPBIR (Integrated Primate Biomaterials and Information Resource). Agarose-embedded high molecularweight DNA was partially digested with a combination of EcoRI restriction enzyme and EcoRI methylase. Size fractionatedDNA was ligated into the pTARBAC2.1 vector between the EcoRI sites. The ligation products were transformed into DH10B (T1resistant) electrocompetent cells (Invitrogen). The library has been arrayed into 528 384-well microtiter dishes and alsogridded onto 11 22x22cm nylon high-densityfilter for screening by probe hybridization. Each hybridizationmembrane represents over 18,000 distinct BAC clones, stamped in duplicate. BAC library construction was funded by NIH grantsHG01165-07SI and HG025323-01 as part of the NIH-fundedBAC Resource Network.
Provisional Data for Marmoset BAC library:
| Segment | Cloning Vector | DNA | Restriction Enzyme | Plate Numbers | Total Plates | Empty Wells | Empty Wells (%) | | 1 | pTARBAC2.1 | Adult Male kidney | EcoRI | 1-288 | 288 | 1,506 | 1.4 | | 2 | pTARBAC2.1 | Adult Male kidney | EcoRI | 289-528 | 240 | 1,071 | 1.2 | | Total Library | | | | 1-528 | 569 | 2,577 | 1.3 |
| Segment | Non-insert Clones (%) | Non-insert clones | Non-recombinant clones (BAC vector) | Recombinant Clones | Average Insert size (Kbp) | Redundancy (Genome size:3.43 Gb) | | 1 | <1% | N/A | N/A | N/A | 170 | N/A | | 2 | | N/A | N/A | N/A | 170 | N/A | | Total Library | <1% | N/A | N/A | N/A | 170 | N/A |
click here for a legend of the previous tables.
The CHORI-259 clone average insert size has been determined by Pulsed Field Gel Electrophoresis. Clone size distribution will be plotted as soon as available.
The library is available as library plates, individual clones, and high-density hybridization sets (see Filter Availability). Individual clones identified after screening can be obtained as stab cultures at a cost of $80 per clone in addition to a $35 set-up charge per shipment. Shipping will be done by Federal Express and charged to the recipients account number.
With respect to the scheduling of shipments of arrayed library copies, we would like to make you aware of our policy for array distribution.
Please visit the ordering information page if you want to place an order.
Academic and commercial users interested in a copy of the BAC library should contact Pieter J. de Jong ([email protected], fax: (510) 450-7924). For hybridization membranes, please contact BACPAC Resources ([email protected]).
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