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CHORI-36: Mouse c57bl/6j (M) (Mus musculus) BAC Library |
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The CHORI-36 c57bl/6j mouse (Mus musculus) BAC library has been constructed by Dr. Michael Nefedov in Pieter De Jong's Laboratory at BACPAC Resources, Children’s Hospital Oakland Research Institute. The preparation of the library followed the cloning approach developed in our laboratory Osoegawa et al., 1998. The male mouse sample was obtained through the Jackson Laboratory. Spleen and brain genomic DNA samples were isolated and partially digested with EcoRI in the presence of EcoRI methylase. Size-selected EcoRI fragments were cloned into the pTARBAC2.1 vector between the EcoRI sites. The ligation products were transformed into DH10B (T1 phage resistant) electrocompetent cells (Invitrogen). The library has been arrayed into 384-well microtiter dishes. The 22x22cm nylon high-density filters have been made available for probe hybridization screening. Library characterization was performed by Qing Cao and Dr. Kazutoyo Osoegawa.
BAC library construction was funded by NIH grants HG01165-07SI and HG025323-01 as part of the NIH-funded BAC Resource Network .
Provisional data for CHORI-36 Mouse c57bl/6j (M) (Mus musculus) BAC Library:
Segment | 1 | 2 | All | Vector | pTARBAC2.1 | pTARBAC2.1 | pTARBAC2.1 | Restriction Enzyme | EcoRI/EcoRI Methylase | EcoRI/EcoRI Methylase | EcoRI/EcoRI Methylase | DNA Source | N/A | N/A | N/A | Plate Numbers | 1-288 | 289-528 | 1-528 | Plate Count | 288 | 240 | 528 | Empty Wells | 4030 (3.64%) | 2617 (2.84%) | 6647 (3.28%) | Non-Recombinant Clones | N/A | N/A | N/A | Non-Insert Clones | Approx. 0 (0/268, 0%) | Approx. 6760 (16/212, 7.55%) | Approx. 6530 (16/480, 3.33%) | Recombinant Clones | 106562 | 82783 | 189575 | Average Insert Size | 173 Kbp | 182 Kbp | N/A | Genomic Coverage | N/A | N/A | N/A |
Click here for legend of the previous table.
No clones were found to be non-recombinant after analysis of CHORI-36 using overgo probes specific for the puc19 fragment.
Data on the CHORI-36 clone insert size distribution has been determined by pulsed-field gel Electrophoresis. Clone Size Distribution has been plotted graphically.
Further in depth characterization of the library is on going in our lab and data will be updated on our web page periodically.
Please direct questions concerning this library to either Pieter J. de Jong.
Ordering & Pricing information
The library is available in several formats. Individual clones, and high-density hybridization filter are obtainable. For ordering and shipping details, please view the ordering and pricing information ordering and pricing information page.
Academic and commercial users interested in a copy of the BAC library should contact Pieter J. de Jong ([email protected], fax: (510) 450-7924).
Reference
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