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 Home > Resources > Libraries
   CHORI-1277: Female Gorilla "Kamilah" (Gorilla gorilla gorilla) Fosmid Library   

Background information

The CHORI-277 Gorilla Fosmid Library has been generated by Baoli Zhu, in Pieter de Jong’s laboratory at BACPAC Resources, Children’s Hospital Oakland Research Institute. The library was prepared from sheared genomic DNA, end-repaired and then size-selected for ~40 kb using pulsed-field electrophoresis. Size-purified DNA was cloned into the dephosphorylated fosmid vector pFOSCC1 (Epicenter).

Blood from female gorilla “Kamilah” was kindly provided by Dr. Oliver Ryder from the Zoological Society of San Diego's center for Conservation and Research for Endangered Species (CRES). White blood cells were embedded in 0.5% InCert agarose and proteins were removed by a detergent/proteinaseK treatment. The resulting agarose-embedded high molecular weight DNA was isolated from agarose after melting and treatment with agarase prior to shearing the DNA to the correct size. The fosmid library has been arrayed into 576, 384-well microtiter dishes.

The preparation of this library was facilitated through a subcontract from the Sanger Institute, funded by The Wellcome Trust. The fosmid library will be used along with the BAC library as key reagents for gorilla genome project. BAC-, fosmid- and small-insert plasmid derived data will be co-assembled with each derived from the same gorilla "Kamilah". The library is in the public domain. About 418,000 Fosmid-end-sequences can be found in the NCBI Trace Archive using the following query string: [species_code="GORILLA GORILLA" and center_name="SC" and strategy="CLONEEND" and library_ID="CHORI-1277"].

Segment

1

All

Vector

pCC1FOS

pCC1FOS

Restriction Enzyme

N/A

N/A

DNA Source

N/A

N/A

Plate Numbers

1-576

1-576

Plate Count

576

576

Empty Wells

N/A

N/A

Non-Recombinant Clones

N/A

N/A

Non-Insert Clones

N/A

N/A

Recombinant Clones

221184

221184

Average Insert Size

N/A

N/A

Genomic Coverage

N/A

N/A

Please address questions concerning this library to Pieter J. de Jong.

Reference

Scally A, Dutheil JY, Hillier LW, Jordan GE, Goodhead I, et al. (2012) Insights into hominid evolution from the gorilla genome sequence. Nature 483: 169-175.

 

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