The CHORI-25 Mouse c57bl/6j Mus musculus Sheared BAC library has been constructed by Kazutoyo Osoegawa in Pieter De Jong's Laboratory at BACPAC Resources, Children’s Hospital Oakland Research Institute. Mouse liver genomic DNA samples were isolated and sheared by repeating freeze and thaw cycles. The fragmented ends were polished by T4 DNA polymerase. The polished ends were ligated to the blunt-end side of an adapter which has a 3'overhang. Size fractionated DNA was cloned into the pTARBAC6 vector between the BstXI sites. The ligation products were transformed into DH10B T1 phage resistant electrocompetent cells (Invitrogen). The library has been arrayed into 672 (384-well) microtiter dishes. The 22x22cm nylon high-density filters have been made available for probe hybridization screening. Library characterization was performed by Teresa Ren, Qing Cao and Kazutoyo Osoegawa.
Provisional data for CHORI-25 Mouse c57bl/6j (F) (Mus musculus) Sheared BAC Library :
No clones were found to be non-recombinant after analysis of CHORI-25 using overgo probes specific for the puc19 fragment.
Data on the CHORI-25 clone insert size distribution has been determined by pulsed-field gel Electrophoresis. Clone Size Distribution has been plotted graphically.
Further in depth characterization of the library is on going in our lab and data will be updated on our web page periodically.
Please direct questions concerning this library to either Pieter J. de Jong.Ordering & Pricing information The library is available in several formats. Individual clones, and high-density hybridization filter are obtainable. For ordering and shipping details, please clickhere.
Academic and commercial users interested in a copy of the BAC library should contact Pieter J. de Jong ([email protected], fax: (510) 450-7924).
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