Cloning site: MboI (GATC); insert is underlined

 

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TACATTATACGAAGTTATATTCGATGCGGCCGCTAATACGACTCACTATAGGGAGAAGCTTGGATC NNNNNNNNNNNNNNNNGATCCTATAGTGTCACCTAAATCGTATGCGGCCGCCCGGGCCGTCGACCA

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����� �1� GATCCTATAG TGTCACCTAA ATCGTATGCG GCCGCCCGGG CCGTCGACCA

����� 51� ATTCTCATGT TTGACAGCTT ATCATCGAAT TTCTGCCATT CATCCGCTTA

���� 101� TTATCACTTA TTCAGGCGTA GCAACCAGGC GTTTAAGGGC ACCAATAACT

���� 151� GCCTTAAAAA AATTACGCCC CGCCCTGCCA CTCATCGCAG TACTGTTGTA

���� 201� ATTCATTAAG CATTCTGCCG ACATGGAAGC CATCACAAAC GGCATGATGA

���� 251� ACCTGAATCG CCAGCGGCAT CAGCACCTTG TCGCCTTGCG TATAATATTT

���� 301� GCCCATGGTG AAAACGGGGG CGAAGAAGTT GTCCATATTG GCCACGTTTA

���� 351� AATCAAAACT GGTGAAACTC ACCCAGGGAT TGGCTGAGAC GAAAAACATA

���� 401� TTCTCAATAA ACCCTTTAGG GAAATAGGCC AGGTTTTCAC CGTAACACGC

���� 451� CACATCTTGC GAATATATGT GTAGAAACTG CCGGAAATCG TCGTGGTATT

���� 501� CACTCCAGAG CGATGAAAAC GTTTCAGTTT GCTCATGGAA AACGGTGTAA

���� 551� CAAGGGTGAA CACTATCCCA TATCACCAGC TCACCGTCTT TCATTGCCAT

���� 601� ACGGAATTCC GGATGAGCAT TCATCAGGCG GGCAAGAATG TGAATAAAGG

���� 651� CCGGATAAAA CTTGTGCTTA TTTTTCTTTA CGGTCTTTAA AAAGGCCGTA

���� 701� ATATCCAGCT GAACGGTCTG GTTATAGGTA CATTGAGCAA CTGACTGAAA

���� 751� TGCCTCAAAA TGTTCTTTAC GATGCCATTG GGATATATCA ACGGTGGTAT

���� 801� ATCCAGTGAT TTTTTTCTCC ATTTTAGCTT CCTTAGCTCC TGAAAATCTC

���� 851� GATAACTCAA AAAATACGCC CGGTAGTGAT CTTATTTCAT TATGGTGAAA

���� 901� GTTGGAACCT CTTACGTGCC GATCAACGTC TCATTTTCGC CAAAAGTTGG

���� 951� CCCAGGGCTT CCCGGTATCA ACAGGGACAC CAGGATTTAT TTATTCTGCG

��� 1001� AAGTGATCTT CCGTCACAGG TATTTATTCG CGATAAGCTC ATGGAGCGGC

��� 1051� GTAACCGTCG CACAGGAAGG ACAGAGAAAG CGCGGATCTG GGAAGTGACG

��� 1101� GACAGAACGG TCAGGACCTG GATTGGGGAG GCGGTTGCCG CCGCTGCTGC

��� 1151� TGACGGTGTG ACGTTCTCTG TTCCGGTCAC ACCACATACG TTCCGCCATT

��� 1201� CCTATGCGAT GCACATGCTG TATGCCGGTA TACCGCTGAA AGTTCTGCAA

��� 1251� AGCCTGATGG GACATAAGTC CATCAGTTCA ACGGAAGTCT ACACGAAGGT

��� 1301� TTTTGCGCTG GATGTGGCTG CCCGGCACCG GGTGCAGTTT GCGATGCCGG

��� 1351� AGTCTGATGC GGTTGCGATG CTGAAACAAT TATCCTGAGA ATAAATGCCT

��� 1401� TGGCCTTTAT ATGGAAATGT GGAACTGAGT GGATATGCTG TTTTTGTCTG

��� 1451� TTAAACAGAG AAGCTGGCTG TTATCCACTG AGAAGCGAAC GAAACAGTCG

��� 1501� GGAAAATCTC CCATTATCGT AGAGATCCGC ATTATTAATC TCAGGAGCCT

��� 1551� GTGTAGCGTT TATAGGAAGT AGTGTTCTGT CATGATGCCT GCAAGCGGTA

��� 1601� ACGAAAACGA TTTGAATATG CCTTCAGGAA CAATAGAAAT CTTCGTGCGG

��� 1651� TGTTACGTTG AAGTGGAGCG GATTATGTCA GCAATGGACA GAACAACCTA

��� 1701� ATGAACACAG AACCATGATG TGGTCTGTCC TTTTACAGCC AGTAGTGCTC

��� 1751� GCCGCAGTCG AGCGACAGGG CGAAGCCCTC GAGTGAGCGA GGAAGCACCA

��� 1801� GGGAACAGCA CTTATATATT CTGCTTACAC ACGATGCCTG AAAAAACTTC

��� 1851� CCTTGGGGTT ATCCACTTAT CCACGGGGAT ATTTTTATAA TTATTTTTTT

��� 1901� TATAGTTTTT AGATCTTCTT TTTTAGAGCG CCTTGTAGGC CTTTATCCAT

��� 1951� GCTGGTTCTA GAGAAGGTGT TGTGACAAAT TGCCCTTTCA GTGTGACAAA

��� 2001� TCACCCTCAA ATGACAGTCC TGTCTGTGAC AAATTGCCCT TAACCCTGTG

��� 2051� ACAAATTGCC CTCAGAAGAA GCTGTTTTTT CACAAAGTTA TCCCTGCTTA

��� 2101� TTGACTCTTT TTTATTTAGT GTGACAATCT AAAAACTTGT CACACTTCAC

��� 2151� ATGGATCTGT CATGGCGGAA ACAGCGGTTA TCAATCACAA GAAACGTAAA

��� 2201� AATAGCCCGC GAATCGTCCA GTCAAACGAC CTCACTGAGG CGGCATATAG

��� 2251� TCTCTCCCGG GATCAAAAAC GTATGCTGTA TCTGTTCGTT GACCAGATCA

��� 2301� GAAAATCTGA TGGCACCCTA CAGGAACATG ACGGTATCTG CGAGATCCAT

��� 2351� GTTGCTAAAT ATGCTGAAAT ATTCGGATTG ACCTCTGCGG AAGCCAGTAA

��� 2401� GGATATACGG CAGGCATTGA AGAGTTTCGC GGGGAAGGAA GTGGTTTTTT

��� 2451� ATCGCCCTGA AGAGGATGCC GGCGATGAAA AAGGCTATGA ATCTTTTCCT

��� 2501� TGGTTTATCA AACGTGCGCA CAGTCCATCC AGAGGGCTTT ACAGTGTACA

��� 2551� TATCAACCCA TATCTCATTC CCTTCTTTAT CGGGTTACAG AACCGGTTTA

��� 2601� CGCAGTTTCG GCTTAGTGAA ACAAAAGAAA TCACCAATCC GTATGCCATG

��� 2651� CGTTTATACG AATCCCTGTG TCAGTATCGT AAGCCGGATG GCTCAGGCAT

��� 2701� CGTCTCTCTG AAAATCGACT GGATCATAGA GCGTTACCAG CTGCCTCAAA

��� 2751� GTTACCAGCG TATGCCTGAC TTCCGCCGCC GCTTCCTGCA GGTCTGTGTT

��� 2801� AATGAGATCA ACAGCAGAAC TCCAATGCGC CTCTCATACA TTGAGAAAAA

� ��2851� GAAAGGCCGC CAGACGACTC ATATCGTATT TTCCTTCCGC GATATCACTT

��� 2901� CCATGACGAC AGGATAGTCT GAGGGTTATC TGTCACAGAT TTGAGGGTGG

��� 2951� TTCGTCACAT TTGTTCTGAC CTACTGAGGG TAATTTGTCA CAGTTTTGCT

��� 3001� GTTTCCTTCA GCCTGCATGG ATTTTCTCAT ACTTTTTGAA CTGTAATTTT

��� 3051� TAAGGAAGCC AAATTTGAGG GCAGTTTGTC ACAGTTGATT TCCTTCTCTT

��� 3101� TCCCTTCGTC ATGTGACCTG ATATCGGGGG TTAGTTCGTC ATCATTGATG

��� 3151� AGGGTTGATT ATCACAGTTT ATTACTCTGA ATTGGCTATC CGCGTGTGTA

��� 3201� CCTCTACCTG GAGTTTTTCC CACGGTGGAT ATTTCTTCTT GCGCTGAGCG

��� 3251� TAAGAGCTAT CTGACAGAAC AGTTCTTCTT TGCTTCCTCG CCAGTTCGCT

��� 3301� CGCTATGCTC GGTTACACGG CTGCGGCGAG CGCTAGTGAT AATAAGTGAC

��� 3351� TGAGGTATGT GCTCTTCTTA TCTCCTTTTG TAGTGTTGCT CTTATTTTAA

��� 3401� ACAACTTTGC GGTTTTTTGA TGACTTTGCG ATTTTGTTGT TGCTTTGCAG

��� 3451� TAAATTGCAA GATTTAATAA AAAAACGCAA AGCAATGATT AAAGGATGTT

��� 3501� CAGAATGAAA CTCATGGAAA CACTTAACCA GTGCATAAAC GCTGGTCATG

��� 3551� AAATGACGAA AGCTATCGCC ATTGCACAGT TTAATGATGA CAGCCCGGAA

��� 3601� GCGAGGAAAA TAACCCGGCG CTGGAGAATA GGTGAAGCAG CGGATTTAGT

��� 3651� TGGGGTTTCT TCTCAGGCTA TCAGAGATGC CGAGAAAGCA GGGCGACTAC

��� 3701� CGCACCCGGA TATGGAAATT CGAGGACGGG TTGAGCAACG TGTTGGTTAT

��� 3751� ACAATTGAAC AAATTAATCA TATGCGTGAT GTGTTTGGTA CGCGATTGCG

��� 3801� ACGTGCTGAA GACGTATTTC CACCGGTGAT CGGGGTTGCT GCCCATAAAG

��� 3851� GTGGCGTTTA CAAAACCTCA GTTTCTGTTC ATCTTGCTCA GGATCTGGCT

��� 3901� CTGAAGGGGC TACGTGTTTT GCTCGTGGAA GGTAACGACC CCCAGGGAAC

��� 3951� AGCCTCAATG TATCACGGAT GGGTACCAGA TCTTCATATT CATGCAGAAG

��� 4001� ACACTCTCCT GCCTTTCTAT CTTGGGGAAA AGGACGATGT CACTTATGCA

��� 4051� ATAAAGCCCA CTTGCTGGCC GGGGCTTGAC ATTATTCCTT CCTGTCTGGC

��� 4101� TCTGCACCGT ATTGAAACTG AGTTAATGGG CAAATTTGAT GAAGGTAAAC

��� 4151� TGCCCACCGA TCCACACCTG ATGCTCCGAC TGGCCATTGA AACTGTTGCT

��� 4201� CATGACTATG ATGTCATAGT TATTGACAGC GCGCCTAACC TGGGTATCGG

��� 4251� CACGATTAAT GTCGTATGTG CTGCTGATGT GCTGATTGTT CCCACGCCTG

��� 4301� CTGAGTTGTT TGACTACACC TCCGCACTGC AGTTTTTCGA TATGCTTCGT

��� 4351� GATCTGCTCA AGAACGTTGA TCTTAAAGGG TTCGAGCCTG ATGTACGTAT

��� 4401� TTTGCTTACC AAATACAGCA ATAGTAATGG CTCTCAGTCC CCGTGGATGG

��� 4451� AGGAGCAAAT TCGGGATGCC TGGGGAAGCA TGGTTCTAAA AAATGTTGTA

��� 4501� CGTGAAACGG ATGAAGTTGG TAAAGGTCAG ATCCGGATGA GAACTGTTTT

��� 4551� TGAACAGGCC ATTGATCAAC GCTCTTCAAC TGGTGCCTGG AGAAATGCTC

��� 4601� TTTCTATTTG GGAACCTGTC TGCAATGAAA TTTTCGATCG TCTGATTAAA

��� 4651� CCACGCTGGG AGATTAGATA ATGAAGCGTG CGCCTGTTAT TCCAAAACAT

��� 4701� ACGCTCAATA CTCAACCGGT TGAAGATACT TCGTTATCGA CACCAGCTGC

��� 4751� CCCGATGGTG GATTCGTTAA TTGCGCGCGT AGGAGTAATG GCTCGCGGTA

��� 4801� ATGCCATTAC TTTGCCTGTA TGTGGTCGGG ATGTGAAGTT TACTCTTGAA

��� 4851� GTGCTCCGGG GTGATAGTGT TGAGAAGACC TCTCGGGTAT GGTCAGGTAA

��� 4901� TGAACGTGAC CAGGAGCTGC TTACTGAGGA CGCACTGGAT GATCTCATCC

��� 4951� CTTCTTTTCT ACTGACTGGT CAACAGACAC CGGCGTTCGG TCGAAGAGTA

��� 5001� TCTGGTGTCA TAGAAATTGC CGATGGGAGT CGCCGTCGTA AAGCTGCTGC

��� 5051� ACTTACCGAA AGTGATTATC GTGTTCTGGT TGGCGAGCTG GATGATGAGC

��� 5101� AGATGGCTGC ATTATCCAGA TTGGGTAACG ATTATCGCCC AACAAGTGCT

��� 5151� TATGAACGTG GTCAGCGTTA TGCAAGCCGA TTGCAGAATG AATTTGCTGG

��� 5201� AAATATTTCT GCGCTGGCTG ATGCGGAAAA TATTTCACGT AAGATTATTA

��� 5251� CCCGCTGTAT CAACACCGCC AAATTGCCTA AATCAGTTGT TGCTCTTTTT

��� 5301� TCTCACCCCG GTGAACTATC TGCCCGGTCA GGTGATGCAC TTCAAAAAGC

��� 5351� CTTTACAGAT AAAGAGGAAT TACTTAAGCA GCAGGCATCT AACCTTCATG

��� 5401� AGCAGAAAAA AGCTGGGGTG ATATTTGAAG CTGAAGAAGT TATCACTCTT

��� 5451� TTAACTTCTG TGCTTAAAAC GTCATCTGCA TCAAGAACTA GTTTAAGCTC

��� 5501� ACGACATCAG TTTGCTCCTG GAGCGACAGT ATTGTATAAG GGCGATAAAA

��� 5551� TGGTGCTTAA CCTGGACAGG TCTCGTGTTC CAACTGAGTG TATAGAGAAA

��� 5601� ATTGAGGCCA TTCTTAAGGA ACTTGAAAAG CCAGCACCCT GATGCGACCA

��� 5651� CGTTTTAGTC TACGTTTATC TGTCTTTACT TAATGTCCTT TGTTACAGGC

��� 5701� CAGAAAGCAT AACTGGCCTG AATATTCTCT CTGGGCCCAC TGTTCCACTT

��� 5751� GTATCGTCGG TCTGATAATC AGACTGGGAC CACGGTCCCA CTCGTATCGT

��� 5801� CGGTCTGATT ATTAGTCTGG GACCACGGTC CCACTCGTAT CGTCGGTCTG

��� 5851� ATTATTAGTC TGGGACCACG GTCCCACTCG TATCGTCGGT CTGATTATCA

��� 5901� GTCTGGAACC ACGGTCCCAC TCGTATCGTC GGTCTGATTA TTAGTCTGGG

��� 5951� ACCACGGTCC CACTCGTATC GTCGGTCTGA TTATTAGTCT GGGACCACGA

��� 6001� TCCCACTCGT GTTGTCGGTC TGATTATCGG TCTGGGACCA CGGTCCCACT

� ��6051� TGTATTGTCG ATCAGACTAT CAGCGTGAGA CTACGATTCC ATCAATGCCT

��� 6101� GTCAAGGGCA AGTATTGACA TGTCGTCGTA ACCTGTAGAA CGGAGTAACC

��� 6151� TCGGTGTGCG GTTGTATGCC TGCTGTGGAT TGCTGCTGTG TCCTGCTTAT

��� 6201� CCACAACATT TTGCGCACGG TTATGTGGAC AAAATACCTG GTTACCCAGG

��� 6251� CCGTGCCGGC ACGTTAACCG GGCTGCATCC GATGCAAGTG TGTCGCTGTC

��� 6301� GACGAGCTCG CGAGCTCGGA CATGAGGTTG CCCCGTATTC AGTGTCGCTG

��� 6351� ATTTGTATTG TCTGAAGTTG TTTTTACGTT AAGTTGATGC AGATCAATTA

��� 6401� ATACGATACC TGCGTCATAA TTGATTATTT GACGTGGTTT GATGGCCTCC

��� 6451� ACGCACGTTG TGATATGTAG ATGATAATCA TTATCACTTT ACGGGTCCTT

��� 6501� TCCGGTGATC CGACAGGTTA CGGGGCGGCG ACCTCGCGGG TTTTCGCTAT

��� 6551� TTATGAAAAT TTTCCGGTTT AAGGCGTTTC CGTTCTTCTT CGTCATAACT

��� 6601� TAATGTTTTT ATTTAAAATA CCCTCTGAAA AGAAAGGAAA CGACAGGTGC

��� 6651� TGAAAGCGAG CTTTTTGGCC TCTGTCGTTT CCTTTCTCTG TTTTTGTCCG

��� 6701� TGGAATGAAC AATGGAAGTC CGAGCTCATC GCTAATAACT TCGTATAGCA

��� 6751� TACATTATAC GAAGTTATAT TCGATGCGGC CGCTAATACG ACTCACTATA

��� 6801� GGGAGAAGCT TGGATC