| Legend
for BAC libraries characterization tables |
 |
Non-recombinant clones in the library containing the intact
vector were identified and distinguished from clones containing
insert by colony hybridization using the vector as a probe.
Non-recombinant clones contain the high-copy number pUC replicon,
lacking in the recombinant clones, thus resulting in a much
higher hybridization signal of vector sequences.
While analyzing BAC clones using pulsed-field gel electrophoresis
to determine the average insert size, non-insert clones containing
a small deleted vector fragment consistent with sucrose resistance
were observed.
The following example is for a different library (CHORI-201
segment 2):
Total number of wells: 288 (total number of plates) x 384
(wells per plate) = 110,592
Total number of clones: 110,592 - 2638 (the number of empty
wells) = 107,954
Approximate number of non-insert clones: 107,954 x 0.022 =
2,375
Number of non-recombinant clones containing the intact vector:
0
A total number of recombinant clones: 107,954 - 2375 - 0 =
105,579
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