|
RPCI-43: Male Chimpanzee (Pan troglodytes) BAC Library |
 |
The RPCI-43 BAC Library has beenconstructed in our laboratory by Chung-Li Shu. DNA was isolated from white blood cells obtained from a male chimpanzee (Pantroglodytes, "Donald", blood collection date: 9-11-97). The blood sample from Donald (# CO551, birthdate: 10-15-1987) was obtained from the Yerkes Non-Human Primate Center in Atlanta, GA. The agarose-embedded DNA was partially digested with a combination of EcoRI and EcoRI Methylase. Size selected DNA was cloned into the pBACe3.6 vector between the EcoRI sites. The ligation products were transformed into DH10B electrocompetent cells (BRL Life Technologies). The library has been arrayed into 384-well microtiter dishes and also gridded onto 22x22cm nylon high-density filters for screening by probe hybridization. Each hybridization membrane represents over 18,000 distinct chimpanzee BAC clones, stamped in duplicate.
The Total RPCI-43Male Chimpanzee BAC Library:
| Segment | Cloning Vector | DNA | Plate Numbers | Total Plates | Total Clones | Total Empty Wells | | 1 | pBACe3.6 | Male WBC | 1-185 | 185 | 68721 | 2319 |
| Segment | (%) Empty Wells | Total Non-Recombinant Clones | % Non-Recombinant Clones | Average Insert Size (Kb) | Genomic Coverage | | 1 | 3.3 | approx. 960 | 1.3 | 157 | 3.5X |
click here for a legend of the previous tables.
The total library should represent approximately 3.5-fold total genomic representation.
The RPCI-43 Male Chimpanzee BAC Library is available both as library plates and high-density hybridization filter sets.
Data on the RPCI-43 clone average insert size has been determined by Pulsed Field Gel Electriphoresis. Clone size distribution has been plotted graphically. Further (in depth) characterization of the library is ongoing in our lab and data will be updated on our web page periodically.
The library is available as library plates, individual clones, and high-density hybridization sets (see Filter Availability)
Individual clones identified after screening can be obtained as stab cultures at a cost of $80 per clone in addition to a $35 set-up charge per shipment. Shipping will be done by Federal Express and charged to the recipients account number.
With respect to the scheduling of shipments of arrayed library copies, we would like to make you aware of our policy for array distribution.
Please visit the ordering information page if you want to place an order.
Academic and commercial users interested in a copy of the BAC library should contact Pieter J. de Jong ([email protected], fax: (510) 450-7924). For hybridization membranes, please contact BACPAC Resources ([email protected]).
|
