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-81: Male (Doberman Pinscher) Canine BAC Library |
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The RPCI-81 Canine BAC Library has been constructed ion our laboratory by Robin Li (Stanford University). The library constructed was collaborative effort that included support from the following groups: Emmanual Mignot (Stanford University), Donald Patterson (University of Pennsylvania), Gus Agiurre (Cornell University), Elaine Ostrander (University of Washington, Seattle), and Bernard van Oost (University of Utrecht, the Netherlands).
Male Doberman Pinscher dog blood genomic DNA, partially digested with EcoRI/EcoRI Methylase, was cloned into the pBACe3.6 vector. The ligation products were transformed into DH10B electrocompetent cells (BRL Life Technologies). The library has been arrayed into 384-well microtiter dishes and gridded onto 22x22cm nylon high density hybridization filters for screening purposes.
The Total RPCI-81 Canine BAC Library:
| Cloning Vector | DNA | Plate Numbers | Total Plates | Total Clones Picked | Total Empty Wells | % Empty Wells | | pBACe3.6 | Doberman Pinscher (Blood) | 1-432 | 432 | 165888 | 5473 | 3.3 |
| Total Non-Recombinant Clones | % Non-Recombinant Clones | Total Clones | Average Insert Size (Kb) | Genomic Coverage | | 3318 | 2.0 | 157255 | 155 | 8.1 |
click here for a legend of the previous tables.
The average insert size data and clone size distribution has been determined by Pulsed Field Gel Electriphoresis analyses of random clones chosen from library plates spanning the entire RPCI-81 library. Further (in depth) characterization is ongoing and data will be updated on this WebPage periodically.
The library is available as library plates, individual clones, and high-density hybridization sets (see Filter Availability)
Individual clones identified after screening can be obtained as stab cultures at a cost of $80 per clone in addition to a $35 set-up charge per shipment. Shipping will be done by Federal Express and charged to the recipients account number.
With respect to the scheduling of shipments of arrayed library copies, we would like to make you aware of our policy for array distribution.
Please visit the ordering information page if you want to place an order.
Academic and commercial users interested in a copy of the BAC library should contact Pieter J. de Jong ([email protected], fax: (510) 450-7924). For hybridization membranes, please contact BACPAC Resources ([email protected]).
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