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RPCI-94: Ceanorabditis briggsea BAC Library |
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The content of this web page is a work in progress. Please contact Pieter De Jong for more information on this library, missing from this page.
The BAC library was created from genomic DNA partially-digested with EcoRI. Size-purified genomic DNA fragments were ligated to the EcoRI-cut pBACe3.6 vector, to replace the small (~3 kb)"stuffer" fragment.
Segment | 1 | 2 | All | Vector | pBACe3.6 | pBACe3.6 | pBACe3.6 | Restriction Enzyme | EcoRI | EcoRI | EcoRI | DNA Source | ? | ? | ? | Plate Numbers | 1-18 | 19-48 | 1-48 | Plate Count | 18 | 30 | 48 | Empty Wells | N/A | N/A | N/A | Non-Recombinant Clones | N/A | N/A | N/A | Non-Insert Clones | N/A | N/A | N/A | Recombinant Clones | 6912 | 11520 | 18432 | Average Insert Size | N/A | N/A | N/A | Genomic Coverage | N/A | N/A | N/A |
Please note that most of the BACs in this library have been BAC-end sequenced. We have mapped the BAC-end sequences (BES) to the most recent assembly (WUGSC1.0/cb3) using the UCSC BLAT program on a local server. A track file has been generated and can be uploaded to the corresponding UCSC C.briggsae assembly by Copy/Past of the following URL:
https://bacpacresources.org/download/rpci94_cb3/RPCI94.bed.
Please note that the mapped BAC-inserts do not start & end with EcoRI sites, as one may predict from the cloning strategy. The reason is that the sequencing primers [used to to generate the BAC-end sequences] were close to the insert/vector junction such that it was impossible to obtain good sequences for the very ends on the insert fragments. One can easily check and find out that there are genomic EcoRI sites about 30-40 bp upstream and downstream of the mapped inserts. These are the predicted true ends of the cloned fragments.
Content Posted: Sep 30th 2003; Updated: October 29, 2010.
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