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RPCI-99: Chinese Hamster Ovary (Cricetulus Griseus) BAC Library |
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This library has been constructed in our laboratory by Chung-Li Shu on the request of Dr. Larry Thompson at Lawrence Livermore National Laboratory in Livermore California. The source DNA was derived from an established cell line "AA8". Reference: Thompson LH, Fong S, Brookman K. Validation of conditions for efficient detection of HPRT and APRT mutations in suspension-cultured Chinese hamster ovary cells. Mutat Res. 1980 Feb;74(1):21-36. This cell line is known to have a highly-rearranged karyotype. DNA was isolated and partially digested with a combination of EcoRI and EcoRI Methylase. Size selected DNA was cloned into the pBACe3.6 vector between the EcoRI sites. The ligation products were transformed into DH10B electrocompetent cells (BRL Life Technologies). The library has been arrayed into 384-well microtiter dishes and also gridded onto 22x22cm nylon high-density filters for screening by probe hybridization. Each hybridization membrane represents over 18,000 distinct bovine BAC clones, stamped in duplicate.For further information, please contact Pieter De Jong
Segment | 1 | All | Vector | pBACe3.6 | pBACe3.6 | Restriction Enzyme | EcoRI/EcoRI Methylase | EcoRI/EcoRI Methylase | DNA Source | ? | ? | Plate Numbers | 1-288 | 1-288 | Plate Count | 288 | 288 | Empty Wells | N/A | N/A | Non-Recombinant Clones | N/A | N/A | Non-Insert Clones | N/A | N/A | Recombinant Clones | 110592 | 110592 | Average Insert Size | 158 Kbp | 158 Kbp | Genomic Coverage | 5.4X | 5.4X |
Content Posted: Sep 30th 2003. Update March 12, 2004
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