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BDGP-DpsF01: Drosophila (Drosophila pseudoobscura) Fosmid Library |
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Genomic fosmid libraries were constructed for the Berkeley Drosophila Genome Project by Andreas Gnirke at Exelixis, Inc. (South San Francisco) from Drosophila stocks obtained from various sources. D. erecta and D. pseudoobscura stocks were obtained from the Drosophila species stock center, which has relocated to Tuscon (http://stockcenter.arl.arizona.edu/). The D. littoralis stock was obtained from the Umea, Sweden stock center via Kevin White (Yale). The Umea stock center is now closed. The D. willistoni stock was obtained from Jeffrey Powell (Yale). The species identifications of the stocks were confirmed by David Grimaldi (American Museum of Natural History, NY). To make the libraries, genomic DNA was partially digested with MboI, size-selected by pulsed-field-gel electrophoresis and cloned in the BamHI site of the fosmid vector pFOS1 (U.-J. Kim et al. (1992) Nucleic Acids Res. 20: 1083 -1085) in the E. coli host strain XL1-Blue MR (Stratagene).
library species stock information clone nomenclature
DERF01 D. erecta Tucson #14021-0224.0 DER36E01 (Plate Information)
DPSF01 D. pseudoobscura Tucson #14011-0121.4 DPS24A05 (Plate Information)
DVIF01 D. virilis* [Umea #S060 (Kevin White) DLI14B24] (Plate Information)
DWIF01 D. willistoni Guadeloupe (Jeffrey Powell) DWI04E31 (Plate Information)
* The DVIF01 library was previously thought to be derived from D. littoralis and was thus previously known as "DLIF01". Upon sequencing of several clones, it became apparent that a strain mixup might have occurred. Using appropiate fly genetics at the Berkeley Drosophila Genome Project Sequencing Center, the source of the library was unambiguously identified as D.virilis. (Susan Celniker, personal communication)
Link to information on pFOS1
A map of pFOS1 is available there:
Click on "protocols", "construction of BAC libraries", and "pFOS1" to get a
map of the pFOS1 plasmid. To prepare the vector arms the plasmid is
linearized at the AatII site, dephosphorylated and then digested with BamHI.
Please note that the pUC-derived portion of the plasmid (between the two cos
sites) is lost during in vitro packaging.
Vector sequence information
Link to Drosophila species stock center in Tucson, AZ
Individual clones identified after screening can be obtained as stab cultures at a cost of $80 per clone in addition to a $35 set-up charge per shipment. Shipping will be done by Federal Express and charged to recipients account number.
With respect to the scheduling of shipments of arrayed library copies, we would like to make you aware of our policy for array distribution.
Please visit the ordering information page if you want to place an order.
Academic and commercial users interested in a copy of the BAC library should contact Pieter J. de Jong ([email protected], fax: (510) 450-7924). For hybridization membranes, please contact BACPAC Resources ([email protected]).
Academic and commercial users interested in a copy of the BAC library should contact Pieter J. de Jong [email: pdejong@chori, fax: (510) 540-7924].
With respect to the scheduling of shipments of arrayed library copies, we would like to make you aware of our policy for array distribution.
Please visit the ordering information page if you want to place an order.
Academic and commercial users interested in a copy of the BAC library should contact Pieter J. de Jong ([email protected], fax: (510) 450-7924). For hybridization membranes, please contact BACPAC Resources ([email protected]).
With respect to the scheduling of shipments of arrayed library copies, we would like to make you aware of our policy for array distribution.
Please visit the ordering information page if you want to place an order.
Academic and commercial users interested in a copy of the BAC library should contact Pieter J. de Jong ([email protected], fax: (510) 450-7924). For hybridization membranes, please contact BACPAC Resources ([email protected]).
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